A genome guided evaluation of the Lab4 probiotic consortium

被引：6

作者：

Baker, L. M. ^{[1
]}

Webberley, T. S. ^{[2
]}

Masetti, G. ^{[2
,6
]}

Hughes, T. R. ^{[3
]}

Marchesi, J. R. ^{[4
]}

Jack, A. A. ^{[2
]}

Joyce, T. S. C. ^{[2
]}

Allen, M. D. ^{[2
]}

Plummer, S. F. ^{[2
]}

Michael, D. R. ^{[2
]}

Ramanathan, G. ^{[5
]}

Del Sol, R. ^{[1
]}

Facey, P. D. ^{[1
]}

机构：

[1] Swansea Univ, Med Sch, Singleton Pk Campus, Swansea SA2 8PP, W Glam, Wales

[2] Cultech Ltd, Unit 2 Christchurch Rd,Baglan Ind Pk, Port Talbot SA12 7BZ, Wales

[3] Cardiff Univ, Syst Immun Res Inst, Henry Welcome Bldg, Cardiff CF14 4XN, Wales

[4] Imperial Coll London, Fac Med, Dept Metab Digest & Reprod, Div Digest Dis, London, England

[5] Pennington Biomed Res Ctr, Pharmacol Based Clin Trials, 6400 Perkins Rd, Baton Rouge, LA 70808 USA

[6] Univ Trento, Dept Cellular Computat & Integrat Biol, Povo, Italy

来源：

GENOMICS | 2021年 / 113卷 / 06期

关键词：

Probiotic; Lactobacillus; Bifidobacterium; Lab4; Probiogenomics; Safety; LACTIC-ACID BACTERIA; ANTIBIOTIC-RESISTANCE; BINDING PROTEIN; LACTOBACILLUS; SURFACE; GENES;

D O I：

10.1016/j.ygeno.2021.08.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Draft genome sequences of the Lab4 probiotic consortium were deposited in Genbank: Bifidobacterium animalis subsp lactis CUL34 (PRJNA482550), Bifidobacterium bifidum CUL20 (PRJNA559984), Lactobacillus acidophilus CUL60 (PRJNA482335), Lactobacillus acidophilus CUL21 (PRJNA482434). Probiogenomic analyses confirmed existing taxonomies and identified putative gene sequences that were functionally related to the performance of each organism during in vitro assessments of bile and acid tolerability, adherence to enterocytes and susceptibility to antibiotics. Genomic stability predictions identified no significant risk of gene acquisition of both antibiotic resistance and virulence genes. These observations were supported by acute phase and repeat dose tolerability studies in Wistar rats. High doses of Lab4 did not result in mortalities, clinical/histopathological abnormalities nor systemic toxicity. Increased faecal numbers of Lab4 in supplemented rats implied survival through the gastrointestinal tract and/or impact the intestinal microbiota composition. In summary, this study provides multifaceted support for probiotic functionality and the safety of the Lab4 consortium.

引用

页码：4028 / 4038

页数：11

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