A genome guided evaluation of the Lab4 probiotic consortium

被引：6
作者：
Baker, L. M. [1 ]
Webberley, T. S. [2 ]
Masetti, G. [2 ,6 ]
Hughes, T. R. [3 ]
Marchesi, J. R. [4 ]
Jack, A. A. [2 ]
Joyce, T. S. C. [2 ]
Allen, M. D. [2 ]
Plummer, S. F. [2 ]
Michael, D. R. [2 ]
Ramanathan, G. [5 ]
Del Sol, R. [1 ]
Facey, P. D. [1 ]
机构：
[1] Swansea Univ, Med Sch, Singleton Pk Campus, Swansea SA2 8PP, W Glam, Wales
[2] Cultech Ltd, Unit 2 Christchurch Rd,Baglan Ind Pk, Port Talbot SA12 7BZ, Wales
[3] Cardiff Univ, Syst Immun Res Inst, Henry Welcome Bldg, Cardiff CF14 4XN, Wales
[4] Imperial Coll London, Fac Med, Dept Metab Digest & Reprod, Div Digest Dis, London, England
[5] Pennington Biomed Res Ctr, Pharmacol Based Clin Trials, 6400 Perkins Rd, Baton Rouge, LA 70808 USA
[6] Univ Trento, Dept Cellular Computat & Integrat Biol, Povo, Italy
来源：
GENOMICS | 2021年 / 113卷 / 06期
关键词：
Probiotic; Lactobacillus; Bifidobacterium; Lab4; Probiogenomics; Safety; LACTIC-ACID BACTERIA; ANTIBIOTIC-RESISTANCE; BINDING PROTEIN; LACTOBACILLUS; SURFACE; GENES;
D O I：
10.1016/j.ygeno.2021.08.007
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Draft genome sequences of the Lab4 probiotic consortium were deposited in Genbank: Bifidobacterium animalis subsp lactis CUL34 (PRJNA482550), Bifidobacterium bifidum CUL20 (PRJNA559984), Lactobacillus acidophilus CUL60 (PRJNA482335), Lactobacillus acidophilus CUL21 (PRJNA482434). Probiogenomic analyses confirmed existing taxonomies and identified putative gene sequences that were functionally related to the performance of each organism during in vitro assessments of bile and acid tolerability, adherence to enterocytes and susceptibility to antibiotics. Genomic stability predictions identified no significant risk of gene acquisition of both antibiotic resistance and virulence genes. These observations were supported by acute phase and repeat dose tolerability studies in Wistar rats. High doses of Lab4 did not result in mortalities, clinical/histopathological abnormalities nor systemic toxicity. Increased faecal numbers of Lab4 in supplemented rats implied survival through the gastrointestinal tract and/or impact the intestinal microbiota composition. In summary, this study provides multifaceted support for probiotic functionality and the safety of the Lab4 consortium.
引用
收藏
页码：4028 / 4038
页数：11
相关论文
共 64 条
  • [1] Tetracycline resistance medicated by tet(W), tet(M), and tet(O) genes of Bifidobactefium isolates from humans
    Aires, J.
    Doucet-Populaire, F.
    Butel, M. J.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (08) : 2751 - 2754
  • [2] CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database
    Alcock, Brian P.
    Raphenya, Amogelang R.
    Lau, Tammy T. Y.
    Tsang, Kara K.
    Bouchard, Megane
    Edalatmand, Arman
    Huynh, William
    Nguyen, Anna-Lisa, V
    Cheng, Annie A.
    Liu, Sihan
    Min, Sally Y.
    Miroshnichenko, Anatoly
    Tran, Hiu-Ki
    Werfalli, Rafik E.
    Nasir, Jalees A.
    Oloni, Martins
    Speicher, David J.
    Florescu, Alexandra
    Singh, Bhavya
    Faltyn, Mateusz
    Hernandez-Koutoucheva, Anastasia
    Sharma, Arjun N.
    Bordeleau, Emily
    Pawlowski, Andrew C.
    Zubyk, Haley L.
    Dooley, Damion
    Griffiths, Emma
    Maguire, Finlay
    Winsor, Geoff L.
    Beiko, Robert G.
    Brinkman, Fiona S. L.
    Hsiao, William W. L.
    Domselaar, Gary, V
    McArthur, Andrew G.
    [J]. NUCLEIC ACIDS RESEARCH, 2020, 48 (D1) : D517 - D525
  • [3] A comparison of methods for extracting plasmids from a difficult to lyse bacterium: Lactobacillus casei
    Alimolaei, Mojtaba
    Golchin, Mehdi
    [J]. BIOLOGICALS, 2017, 45 : 47 - 51
  • [4] Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance
    Aquilina, Gabriele
    Bories, Georges
    Chesson, Andrew
    Cocconcelli, Pier Sandro
    de Knecht, Joop
    Dierick, Noel Albert
    Gralak, Mikolaj Antoni
    Gropp, Jurgen
    Halle, Ingrid
    Hogstrand, Christer
    Leng, Lubomir
    Puente, Secundino Lopez
    Haldorsen, Anne-Katrine Lundebye
    Mantovani, Alberto
    Martelli, Giovanna
    Mezes, Miklos
    Renshaw, Derek
    Saarela, Maria
    Sejrsen, Kristen
    Westendorf, Johannes
    [J]. EFSA JOURNAL, 2012, 10 (06)
  • [5] The RAST server: Rapid annotations using subsystems technology
    Aziz, Ramy K.
    Bartels, Daniela
    Best, Aaron A.
    DeJongh, Matthew
    Disz, Terrence
    Edwards, Robert A.
    Formsma, Kevin
    Gerdes, Svetlana
    Glass, Elizabeth M.
    Kubal, Michael
    Meyer, Folker
    Olsen, Gary J.
    Olson, Robert
    Osterman, Andrei L.
    Overbeek, Ross A.
    McNeil, Leslie K.
    Paarmann, Daniel
    Paczian, Tobias
    Parrello, Bruce
    Pusch, Gordon D.
    Reich, Claudia
    Stevens, Rick
    Vassieva, Olga
    Vonstein, Veronika
    Wilke, Andreas
    Zagnitko, Olga
    [J]. BMC GENOMICS, 2008, 9 (1)
  • [6] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
    Bankevich, Anton
    Nurk, Sergey
    Antipov, Dmitry
    Gurevich, Alexey A.
    Dvorkin, Mikhail
    Kulikov, Alexander S.
    Lesin, Valery M.
    Nikolenko, Sergey I.
    Son Pham
    Prjibelski, Andrey D.
    Pyshkin, Alexey V.
    Sirotkin, Alexander V.
    Vyahhi, Nikolay
    Tesler, Glenn
    Alekseyev, Max A.
    Pevzner, Pavel A.
    [J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) : 455 - 477
  • [7] The interaction between bacteria and bile
    Begley, M
    Gahan, CGM
    Hill, C
    [J]. FEMS MICROBIOLOGY REVIEWS, 2005, 29 (04) : 625 - 651
  • [8] Screening for Antimicrobial Resistance Genes and Virulence Factors via Genome Sequencing
    Bennedsen, Mads
    Stuer-Lauridsen, Birgitte
    Danielsen, Morten
    Johansen, Eric
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (08) : 2785 - 2787
  • [9] GenBank
    Benson, Dennis A.
    Cavanaugh, Mark
    Clark, Karen
    Karsch-Mizrachi, Ilene
    Ostell, James
    Pruitt, Kim D.
    Sayers, Eric W.
    [J]. NUCLEIC ACIDS RESEARCH, 2018, 46 (D1) : D41 - D47
  • [10] IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets
    Bertelli, Claire
    Laird, Matthew R.
    Williams, Kelly P.
    Lau, Britney Y.
    Hoad, Gemma
    Winsor, Geoffrey L.
    Brinkman, Fiona S. L.
    [J]. NUCLEIC ACIDS RESEARCH, 2017, 45 (W1) : W30 - W35
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