Size-exclusion chromatography based on silica-diol for the analysis of the proinsulin fusion protein

被引：7
作者：
Gusarova, V. [1 ]
Vorobjeva, T. [1 ]
Gusarov, D. [1 ]
Lasman, V. [1 ]
Bayramashvili, D. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst, Expt Biotechnol Plant, Moscow 117997, Russia
来源：
JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1176卷 / 1-2期
关键词：
size-exclusion chromatography; validation; qualification; proinsulin fusion protein;
D O I：
10.1016/j.chroma.2007.10.090
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Size-exclusion chromatography based on silica-diol sorbent was employed to analyze the recombinant proinsulin fusion protein obtained during the process of refolding and the following ion-exchange purification. The assay was qualified as a control method estimating its accuracy, precision, linearity, limit of detection, limits of quantitation, specificity, and robustness. The results show the reliability for the intended use. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 162
页数：6
相关论文
共 50 条
  • [1] Studies on protein denaturation by size-exclusion chromatography
    Chen, GL
    Li, R
    Li, HR
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 1997, 18 (05): : 701 - 705
  • [2] Evaluation of size-exclusion chromatography and size-exclusion electrochromatography calibration curves
    Vander Heyden, Y
    Popovici, ST
    Schoenmakers, PJ
    JOURNAL OF CHROMATOGRAPHY A, 2002, 957 (02) : 127 - 137
  • [3] Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides
    Shimoyama, Atsuko
    Fujisaka, Aid
    Obika, Satoshi
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 136 : 55 - 65
  • [4] Characterization of AOX by fractionation analysis and size-exclusion chromatography
    Gremm, TJ
    Frimmel, FH
    ACTA HYDROCHIMICA ET HYDROBIOLOGICA, 2000, 28 (04): : 202 - 211
  • [5] Protein refolding at high concentration using size-exclusion chromatography
    Batas, D
    Chaudhuri, JB
    BIOTECHNOLOGY AND BIOENGINEERING, 1996, 50 (01) : 16 - 23
  • [6] Poppe plots for size-exclusion chromatography
    Popovici, ST
    Schoenmakers, PJ
    JOURNAL OF CHROMATOGRAPHY A, 2005, 1073 (1-2) : 87 - 91
  • [7] Size-exclusion chromatography with a multipore column
    Nagata, M
    BUNSEKI KAGAKU, 1998, 47 (11) : 895 - 896
  • [8] Size-exclusion chromatography of perfluorosulfonated ionomers
    Mourey, T. H.
    Slater, L. A.
    Galipo, R. C.
    Koestner, R. J.
    JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (34) : 5801 - 5809
  • [9] Light-scattering and turbidimetric detection of silica colloids in size-exclusion chromatography
    Aspanut, Zarina
    Yamada, Tomomi
    Lim, Lee Wah
    Takeuchi, Toyohide
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (01) : 353 - 359
  • [10] Light-scattering and turbidimetric detection of silica colloids in size-exclusion chromatography
    Zarina Aspanut
    Tomomi Yamada
    Lee Wah Lim
    Toyohide Takeuchi
    Analytical and Bioanalytical Chemistry, 2008, 391 : 353 - 359
12345