RFLP clusters of rifampicin resistant and susceptible Mycobacterium tuberculosis strains in Western province of Sri Lanka

被引:0
作者
Adikaram, Chamila Priyangani [1 ]
Wijesundera, Sandya Sulochana [2 ]
Perera, Jennifer [1 ]
机构
[1] Univ Colombo, Dept Microbiol, Fac Med, Kynsey Rd, Colombo 08, Sri Lanka
[2] Univ Colombo, Dept Biochem & Mol Biol, Fac Med, Colombo, Sri Lanka
基金
美国国家科学基金会;
关键词
Restriction fragment length polymorphism; Mycobacterium tuberculosis; rifampicin; Sri Lanka; FRAGMENT-LENGTH-POLYMORPHISM; EPIDEMIOLOGY; REGION;
D O I
10.3855/jidc.7684
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Introduction: Continuous studies on genetic diversity of Mycobacterium tuberculosis could enhance the awareness on transmission, control and prevention of tuberculosis (TB). In this study, we investigated current genetic diversity of TB and rifampicin resistant TB by, Restriction Fragment Length Polymorphism (RFLP) based on fingerprinting of the IS6110 insertion sequence, in the Western province of Sri Lanka, the famous touristic destination with the highest TB burden in the country. Methodology: Genomic DNA extracted from susceptible and rifampicin resistant TB strains (confirmed for rpoB gene point mutations) were digested with PvuII restriction enzyme, electrophoresed and subjected to Southern transfer. The blots were hybridised with IS6110 probe and visualized using a chemiluminescence detection. Results: The number of copies of IS6110 per isolate varied from 1 to 14. The dendrogram revealed a total of 68 distinct strains among 77 TB isolates and they belonged to nine clusters. Both rifampicin resistant and susceptible strains were distributed in all clusters. This evaluation revealed the absence of genetically identical or strong relatedness between susceptible and resistant isolates. However, clonal expansion was detected in transmission of both TB and rifampicin resistant TB. In addition, the resistant isolates having the novel mutation had no clonal relatedness. Conclusion: This is the first observational study regarding clonal expansion of TB in Sri Lanka. Thus, further investigation on genotypes, clonal expansion and transmission of drug resistance using additional markers would be useful for controlling TB.
引用
收藏
页码:619 / 625
页数:7
相关论文
共 50 条
[41]   Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey [J].
Karagoz, Alper ;
Tutun, Hidayet ;
Altintas, Levent ;
Alanbayi, Umit ;
Yildirim, Dilara ;
Kocak, Nadir .
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 23 :130-134
[42]   Proteomic analysis of drug-susceptible and multidrug-resistant nonreplicating Beijing strains of Mycobacterium tuberculosis cultured in vitro [J].
Saiboonjan, Bhanubong ;
Roytrakul, Sittiruk ;
Sangka, Arunnee ;
Lulitanond, Viraphong ;
Faksri, Kiatichai ;
Namwat, Wises .
BIOCHEMISTRY AND BIOPHYSICS REPORTS, 2021, 26
[43]   Whole genome analysis of extensively drug resistant Mycobacterium tuberculosis strains in Peru [J].
Santos-Lazaro, David ;
Gavilan, Ronnie G. ;
Solari, Lely ;
Vigo, Aiko N. ;
Puyen, Zully M. .
SCIENTIFIC REPORTS, 2021, 11 (01)
[44]   Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage [J].
Mendis, Charitha ;
Thevanesam, Vasanthi ;
Kumara, Athula ;
Wickramasinghe, Susiji ;
Madegedara, Dushantha ;
Gamage, Chandika ;
Gordon, Stephen V. ;
Suzuki, Yasuhiko ;
Ratnatunga, Champa ;
Nakajima, Chie .
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2019, 87 :84-91
[45]   Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico [J].
Martinez-Guarneros, Armando ;
Rastogi, Nalin ;
Couvin, David ;
Escobar-Gutierrez, Alejandro ;
Goncalves Rossi, Livia Maria ;
Vazquez-Chacon, Carlos A. ;
Rivera-Gutierrez, Sandra ;
Lozano, Daniela ;
Vergara-Castaneda, Arely ;
Gonzalez-y-Merchand, Jorge A. ;
Vaughan, Gilberto .
INFECTION GENETICS AND EVOLUTION, 2013, 14 :434-443
[46]   Evaluation of a Molecular Test for Detection of Mycobacterium tuberculosis Isolates Resistant to Rifampicin and Isoniazid [J].
Benaissa, E. ;
Benlahlou, Y. ;
Bssaibis, F. ;
Maleb, A. ;
Elouennass, M. .
CLINICAL LABORATORY, 2022, 68 (03) :532-538
[47]   Pharmacophore insights into rpoB gene mutations in Mycobacterium tuberculosis rifampicin resistant isolates [J].
Figueiredo, Ricardo ;
Ramos, Daniela F. ;
Moiteiro, Cristina ;
Medeiros, Maria Augusta ;
Marcelo Curto, Maria Joao ;
de Menezes, Jose Cardoso ;
Hernandez Pando, Rogelio ;
Silva, Pedro E. A. ;
Costa, Maria do Ceu .
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2012, 47 :186-193
[48]   SUSCEPTIBILITY OF RIFAMPICIN-ISONIAZID RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES AGAINST LEVOFLOXACIN [J].
Kurniawan, Alvin Hartanto ;
Mertaniasih, Ni Made ;
Soedarsono .
BALI MEDICAL JOURNAL, 2016, 5 (01) :8-12
[49]   Investigation of Mycobacterium bovis subsp bovis Among the Strains of Mycobacterium tuberculosis Complex Isolated in Duzce Province, Turkey [J].
Ozturk, Cihadiye Elif ;
Sahin, Idris ;
Oksuz, Sukru ;
Kilic, Nida ;
Kilincel, Ozge ;
Aydin, Leyla ;
Atik, Dursun ;
Afsin, Emine .
MIKROBIYOLOJI BULTENI, 2016, 50 (03) :392-400
[50]   Clusters of Multidrug-Resistant Mycobacterium tuberculosis Cases, Europe [J].
Devaux, Isabelle ;
Kremer, Kristin ;
Heersma, Herre ;
Van Socilingen, Dick .
EMERGING INFECTIOUS DISEASES, 2009, 15 (07) :1052-1060