An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricles

被引：53

作者：

Saffer, LD

Gu, RD

Corwin, JT

机构：

[1] UNIV VIRGINIA, SCH MED, DEPT OTOLARYNGOL HNS, CHARLOTTESVILLE, VA 22908 USA

[2] UNIV VIRGINIA, SCH MED, DEPT NEUROSCI, CHARLOTTESVILLE, VA 22908 USA

来源：

HEARING RESEARCH | 1996年 / 94卷 / 1-2期

关键词：

growth factor receptor; reverse transcriptase-polymerase chain reaction; hair cell; regeneration; balance; vestibular;

D O I：

10.1016/0378-5955(95)00228-6

中图分类号：

R36 [病理学]; R76 [耳鼻咽喉科学];

学科分类号：

100104 ; 100213 ;

摘要：

Sensory epithelia from normal rat utricles and those cultured with and without neomycin treatment were assayed for the presence of growth factor receptor mRNAs by RT-PCR (reverse transcriptase-polymerase chain reaction). Both undamaged and damaged utricles showed mRNA for Insulin receptor, IGF-I receptor, FGF receptor 1, EGF receptor, and PDGF alpha receptor. Neomycin-damaged sensory epithelia showed less PDGF alpha receptor mRNA than undamaged epithelia, suggesting that this message may be expressed at higher copy levels in hair cells than in supporting cells. Consistent with that hypothesis, immunohistochemistry revealed much stronger PDGF alpha receptor staining in the hair cells than in the supporting cells. Preliminary evidence suggests that IGF-I receptor message also my be lowered in neomycin-damaged epithelia.

引用

页码：14 / 23

页数：10

共 40 条

[31] EVIDENCE FOR SUPPORTING CELL MITOSIS IN RESPONSE TO ACOUSTIC TRAUMA IN THE AVIAN INNER-EAR [J].