Light emitting diode irradiation protect against the amyloid beta 25-35 induced apoptosis of PC12 cell in vitro

被引：44
作者：
Duan, R
Zhu, L
Liu, TCY [1 ]
Li, Y
Liu, J
Jiao, JL
Xu, XY
Yao, LB
Liu, SH
机构：
[1] S Carolina Normal Univ, Lab Laser Sports Med, Coll Sports Sci, Guangzhou 516031, GD, Peoples R China
[2] Fourth Mil Med Univ, Dept Biochem, Xian 710032, Peoples R China
[3] Huazhong Univ Sci & Technol, State Key Lab Laser Technol, Wuhan 430074, Peoples R China
[4] Minist Educ China, Key Lab Biomed Photon, Wuhan 430074, Peoples R China
[5] Jinan Univ, Coll Med, TCM Dept, Guangzhou 510632, Peoples R China
[6] S China Normal Univ, Sch Informat & Optoelect Sci & Engn, Guangzhou, Peoples R China
来源：
LASERS IN SURGERY AND MEDICINE | 2003年 / 33卷 / 03期
关键词：
Alzheimer's disease; apoptosis; PC12; cells; light emitting diode;
D O I：
10.1002/lsm.10216
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
Background and Objectives: Whether light emitting diode (LED) irradiation has effects on "beta-amyloid (Abeta) induced apoptosis," a leading hypothesis of the cause of Alzheimer's disease (AD), or not? Study Design/Materials and Methods: Monolayer cell cultures of PC12 were subjected to Abeta or/and LED irradiation at various intensity. Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay. Results: Treatment of the cells with LED irradiation significantly diminished Abeta induced apoptosis within 24 hours. Conclusions: The LED irradiation, when utilized at power of 0.9W/m(2) and 60 minutes has significantly diminished Abeta induced apoptosis of PC12 cells. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码：199 / 203
页数：5
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