Efficiency of adenovirus-mediated gene transduction n heart grafts in rats

被引：3
作者：
Fujishiro, J
Kawana, H
Inoue, S
Shimizu, H
Yoshino, H
Hakamata, Y
Kaneko, T
Murakami, T
Hashizume, K
Kobayashi, E
机构：
[1] Jichi Med Sch, Ctr Mol Med, Div Organ Replacement Res, Kawachi, Tochigi 3290498, Japan
[2] Univ Tokyo, Fac Med, Dept Pediat Surg, Tokyo, Japan
来源：
TRANSPLANTATION PROCEEDINGS | 2005年 / 37卷 / 01期
关键词：
D O I：
10.1016/j.transproceed.2004.12.295
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Aim. We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system. Methods. Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals. Results. Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver. Conclusions. We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts.
引用
收藏
页码：67 / 69
页数：3
相关论文
共 9 条
[1]   Tissue-specific consequences of the anti-adenoviral immune response: implications for cardiac transplants [J].
Chan, SY ;
Li, KW ;
Piccotti, JR ;
Louie, MC ;
Judge, TA ;
Turka, LA ;
Eichwald, EJ ;
Bishop, DK .
NATURE MEDICINE, 1999, 5 (10) :1143-1149
[2]   Gene therapy for organ grafts using rapid injection of naked DNA: Application to the rat liver [J].
Inoue, S ;
Hakamata, Y ;
Kaneko, M ;
Kobayashi, E .
TRANSPLANTATION, 2004, 77 (07) :997-1003
[3]   Simultaneous administration of a low-dose mixture of donor bone marrow cells and splenocytes plus adenovirus containing the CTLA41g gene result in stable mixed chimerism and long-term survival of cardiac allograft in rats [J].
Jin, YZ ;
Zhang, QY ;
Hao, B ;
Gao, X ;
Guo, YL ;
Xie, SS .
IMMUNOLOGY, 2003, 110 (02) :275-286
[4]   Adenovirus-mediated gene transfer of the β2-adrenergic receptor to donor hearts enhances cardiac function [J].
Kypson, AP ;
Hendrickson, SC ;
Akhter, SA ;
Wilson, K ;
McDonald, PH ;
Lilly, RE ;
Dolber, PC ;
Glower, DD ;
Lefkowitz, RJ ;
Koch, WJ .
GENE THERAPY, 1999, 6 (07) :1298-1304
[5]   Adenovirus-mediated gene transfer to the rat heart graft [J].
Ogata, Y ;
Fujishiro, J ;
Kawana, H ;
Kobayashi, E .
TRANSPLANTATION, 2004, 78 (09) :1408-1408
[6]   Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex [J].
Okada, T ;
Ramsey, WJ ;
Munir, J ;
Wildner, O ;
Blaese, RM .
NUCLEIC ACIDS RESEARCH, 1998, 26 (08) :1947-1950
[7]   Optical imaging of cardiac reporter gene expression in living rats [J].
Wu, JC ;
Inubushi, M ;
Sundaresan, G ;
Schelbert, HR ;
Gambhir, SS .
CIRCULATION, 2002, 105 (14) :1631-1634
[8]  
Xiu DR, 2001, MICROSURG, V21, P16, DOI 10.1002/1098-2752(2001)21:1<16::AID-MICR1003>3.0.CO
[9]  
2-B
1