Probing the heterogeneous fluorescence lifetime behavior of single rhodamine-6G molecules on fused silica

被引：2

作者：

Ambrose, WP ^{[1
]}

Goodwin, PM ^{[1
]}

Enderlein, J ^{[1
]}

Semin, DJ ^{[1
]}

Keller, RA ^{[1
]}

机构：

[1] Univ Calif Los Alamos Natl Lab, Chem Sci & Technol Div, Los Alamos, NM 87545 USA

来源：

METHODS FOR ULTRASENSITIVE DETECTION | 1998年 / 3270卷

关键词：

single molecule detection; fluorescence lifetime; near-field microscopy; far-field microscopy; fluorescence photon antibunching;

D O I：

10.1117/12.308379

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Heterogeneity in the environments of molecules affects their photophysical parameters. Changes in the local environments or changes within the molecules themselves produces fluctuations in the photophysical parameters. We have explored the fluorescence lifetime and intensity fluctuations of Rhodamine 6G on silica using time correlated single photon counting combined with near-field and far-field scanning optical microscopies, and fluorescence photon-pair correlation measurements in far-field microscopy. In the most recent work, we find complex behavior in the fluorescence lifetime and emission intensity in the millisecond to minute time range that must be associated with changes in both the non-radiative and the radiative decay rates.

引用

页码：190 / 199

页数：10