Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes

被引：30
作者：
Matsuoka, Makoto [1 ]
Akasaka, Tsukasa [2 ]
Totsuka, Yasunori [1 ]
Watari, Fumio [2 ]
机构：
[1] Hokkaido Univ, Grad Sch Dent Med, Dept Oral & Maxillofacial Surg, Kita Ku, Sapporo, Hokkaido 0608586, Japan
[2] Hokkaido Univ, Grad Sch Dent Med, Dept Dent Mat & Engn, Kita Ku, Sapporo, Hokkaido 0608586, Japan
来源：
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS | 2010年 / 173卷 / 1-3期
基金：
日本科学技术振兴机构;
关键词：
Carbon nanotubes; Osteoblast; Adhesion; Vinculin; IN-VITRO; SCAFFOLDS; VIABILITY; TISSUE;
D O I：
10.1016/j.mseb.2009.12.044
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In recent years, carbon nanotubes (CNTs) have been considered potential biomedical materials because of their unique character. The aim of this study was to investigate the response of a human osteoblast-like cell line - Saos-2 - on single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs). The surface of a culture dish was coated with CNTs, and Saos-2 cells were cultured for three days. Cell morphology, viability, alkaline phosphatase (ALP) activity, adhesion, and vinculin expression were evaluated. The result showed high cell viability and strong adhesion to MWCNTs. Saos-2 cultured on MWCNTs exhibited vinculin expression throughout the cell body. while the cells attached to SWCNTs and glass were mostly limited to their periphery. Our results suggest that CNT coatings promote cell activity and adhesiveness. These findings indicate that MWCNTs could be used as surface coating materials to promote cell adhesion. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：182 / 186
页数：5
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