Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model

被引：12
作者：
Lai, Yujie [1 ,2 ]
Hu, Xiaotong [1 ]
Chen, Guojun [1 ]
Wang, Xuefeng [1 ,3 ]
Zhu, Binglin [1 ]
机构：
[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing Key Lab Neurol, 1 Youyi Rd, Chongqing 400016, Peoples R China
[2] Cent S Univ, Xiangya Med Coll, Affiliated Haikou Hosp, Dept Neurol,Haikou Municipal Hosp, Haikou 570208, Hainan, Peoples R China
[3] Beijing Inst Brain Disorders, Ctr Epilepsy, 10 Xitoutiao, Beijing 100069, Peoples R China
来源：
JOURNAL OF THE NEUROLOGICAL SCIENCES | 2016年 / 366卷
基金：
中国国家自然科学基金;
关键词：
Adenylate kinase 5 (AK5); Copine VI (CPNE6); Refractory epilepsy; Patients; Rat model; ATP CHANNEL ACTIVITY; PURINE METABOLISM; NEURONAL-ACTIVITY; SEIZURE ACTIVITY; N-COPINE; EXPRESSION; DEREGULATION;
D O I：
10.1016/j.jns.2016.04.037
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Adenylate kinase 5 (AK5) is one member of the AK family and plays a critical role in maintaining cellular homeostasis. Different from the other AKs, AK5 is almost exclusively expressed in the brain. However, its exact biological functions remain unclear. The aim of the present study is to explore the expression pattern of AK5 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic rat model. Using Western blot, immunofluorescence and immunoprecipitation analysis, we found that AK5 protein was mainly expressed in neurons, demonstrated by colocalization with the dendritic marker, MAP2, which were similar to the corresponding controls. However, the expression of AK5 decreased remarkably in epileptic patients and experimental rats. Furthermore, immunoprecipitation analysis showed that the interaction of AK5 with copine VI (CPNE6, a brain specific protein) increased in epileptic patients and rat models. Our results are the first to indicate that the expression of AK5 in epileptic brain tissue may play important roles in epilepsy, especially refractory epilepsy. (C) 2016 Elsevier B.V. All rights reserved.
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收藏
页码：20 / 26
页数：7
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