Structural and genetic characterization of the O-antigen of Escherichia coli O161 containing a derivative of a higher acidic diamino sugar, legionaminic acid

被引：21
作者：
Li, Xiaomin [2 ]
Perepelov, Andrei V. [3 ]
Wang, Quan [2 ]
Senchenkova, Sof'ya N. [3 ]
Liu, Bin [2 ]
Shevelev, Sergei D. [3 ]
Guo, Xi [2 ]
Shashkov, Alexander S. [3 ]
Chen, Wei [1 ]
Wang, Lei [2 ]
Knirel, Yuriy A. [3 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China
[3] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
来源：
CARBOHYDRATE RESEARCH | 2010年 / 345卷 / 11期
基金：
俄罗斯基础研究基金会; 中国国家自然科学基金;
关键词：
Escherichia coli; O-Polysaccharide structure; O-Antigen gene cluster; 5,7-Diamino-3,5,7,9-tetradeoxynon-2-ulosonic acid; Legionaminic acid; Lipopolysaccharide; PNEUMOPHILA SEROGROUP-1 LIPOPOLYSACCHARIDE; PSEUDAMINIC ACID; LEGIONELLA-PNEUMOPHILA; CAMPYLOBACTER-JEJUNI; FUNCTIONAL-CHARACTERIZATION; 8-EPILEGIONAMINIC ACID; HELICOBACTER-PYLORI; N-ACETYLGLUCOSAMINE; MOLECULAR ANALYSIS; SEQUENCE-ANALYSIS;
D O I：
10.1016/j.carres.2010.04.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-antigen is an essential component of lipopolysaccharide on the surface of Gram-negative bacteria and plays an important role in its pathogenicity. Composition and structure of the O-antigens of Escherichia coli are highly diverse mainly due to genetic variations in the O-antigen gene cluster. In this work, the chemical structure and the gene cluster of the O-antigen of E. coli 0161 were studied. Chemical degradations, sugar analyses, and NMR spectroscopy showed that the 0161 antigen possesses a trisaccharide O-repeating unit containing a 5-N-acetyl-7-N-(D-alanyl) derivative of 5,7-diamino-3,5,7,9-tetradeoxy-oglycero-D-galacto-non-2-ulosonic (legionaminic) acid (Leg5Ac7Ala) and having the following structure: -> 8)-alpha-Legp5Ac7Ala-(2 -> 4)-beta-d-GlcpA-(1 -> 3)-beta-d-GlcpNAc-(1 -> The O-antigen gene cluster of E. coli O161 was sequenced. In addition to the genes encoding sugar transferases, O-repeating unit flippase (Wzx) and O-antigen polymerase (Wzy), the genes involved in the biosynthesis of a legionaminic acid derivative were identified based on database similarities. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1581 / 1587
页数：7
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