A microtiter-plate screening method for biofilm disinfection and removal

被引：265
作者：
Pitts, B
Hamilton, MA
Zelver, N
Stewart, PS [1 ]
机构：
[1] Montana State Univ, Dept Chem Engn, Bozeman, MT 59717 USA
[2] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 USA
[3] Montana State Univ, Dept Math Sci, Bozeman, MT 59717 USA
[4] Montana State Univ, Techlink, Bozeman, MT 59717 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 54卷 / 02期
基金：
美国国家科学基金会;
关键词：
biofilm; microtiter plate; disinfection; removal; spectrophotometric method;
D O I：
10.1016/S0167-7012(03)00034-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：269 / 276
页数：8
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