Isolation and characterization of a Bacillus species capable of producing tetrodotoxin from the puffer fish Fugu obscurus

被引：22
作者：
Wang, Jun [1 ,2 ]
Fan, Yanhui [1 ,2 ]
机构：
[1] Binzhou Univ, Dept Life Sci, Binzhou 256600, Peoples R China
[2] Shandong Key Lab Ecoenvironm Sci Yellow River Del, Binzhou 256600, Peoples R China
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2010年 / 26卷 / 10期
关键词：
Tetrodotoxin; Fugu obscurus; Ovary; Bacillus; STARFISH ASTROPECTEN-POLYACANTHUS; BOSHUBORA CHARONIA-SAULIAE; ATERGATIS-FLORIDUS; TRUMPET SHELL; XANTHID CRAB; BACTERIA; NEUROTOXIN; GASTROPOD; SUBSTANCE; TOXINS;
D O I：
10.1007/s11274-010-0354-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We obtained puffer fish Fugu obscurus from Wudi, China and analyzed the level of tetrodotoxin (TTX) toxictiy by mouse bioassay. The ovary showed the highest potency (125 MU/g), followed by the liver, intestine, and skin. A TTX-producing strain, namely, W-3, was isolated from the ovary of puffer fish F. obscurus. After culturing at 28 A degrees C for 48 h, toxins were extracted from the liquid medium and analyzed by mouse bioassay, high-performance liquid chromatography, and gas chromatography-mass spectrometry. The results showed that strain W-3 produced TTX and related compounds. Physiological and biochemical characterization and 16S rRNA analysis indicated that this strain represents a novel species within the Bacillus genus; we named this strain as Bacillus sp. W-3. Our results suggested that marine bacteria play a role in the production of TTX in puffer fish F. obscurus.
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页码：1755 / 1760
页数：6
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