Upregulated expression of human β defensin-1 and -3 mRNA during differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-0b

被引：28

作者：

Abiko, Y ^{[1
]}

Nishimura, M

Kusano, K

Yamazaki, M

Arakawa, T

Takuma, T

Kaku, T

机构：

[1] Hlth Sci Univ Hokkaido, Dept Oral Pathol, Ishikari, Hokkaido 0610293, Japan

[2] Hlth Sci Univ Hokkaido, Dept Oral Biochem, Sch Dent, Ishikari, Hokkaido 0610293, Japan

来源：

JOURNAL OF DERMATOLOGICAL SCIENCE | 2003年 / 31卷 / 03期

关键词：

beta-defensin; keratinocyte; celt line; differentiation; RT-PCR; antimicrobial peptide;

D O I：

10.1016/S0923-1811(03)00007-0

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background: Human beta-defensins (hBDs) belong to a group of antimicrobial peptide that are expressed in the epithelial cells. Objective: The present study investigated mRNA expression levels of the beta-defensins, hBD-1, -2 and -3, in human keratinocytes during differentiation in vitro. Methods: Immortalized keratinocyte cell lines, HaCaT and PHK16-0b, were used in this study; in order to stimulate differentiation, the Ca2+ concentration in the growth media was increased from 0.3 to 1.8 mM. Results: Four days after the increase, the expression levels of hBD-1 and 3 were increased in both cell lines, followed by an increase in the mRNA levels of the differentiation markers, involucrin and keratin 10. No increased expression of hBD-2 was observed. Conclusion: The results indicate that keratinocyte differentiation may stimulate hBD-1 and -3 expression in stratified squamous epithelia. (C) 2003 Japanese Society for Investigative Dermatology. Published by Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：225 / 228

页数：4

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