A novel fluorescent pH probe for expression in plants

被引：88

作者：

Schulte, Alexander ^{[1
,2
]}

Lorenzen, Inken ^{[2
]}

Boettcher, Markus ^{[3
,4
]}

Plieth, Christoph ^{[1
]}

机构：

[1] Univ Kiel, Zentrum Biochem & Mol Biol, D-24118 Kiel, Germany

[2] Univ Kiel, Inst Biochem, D-24098 Kiel, Germany

[3] Univ Kiel, Inst Bot, D-24118 Kiel, Germany

[4] Med Univ Lubeck, Inst Physiol, D-23538 Lubeck, Germany

来源：

PLANT METHODS | 2006年 / 2卷 / 1期

关键词：

D O I：

10.1186/1746-4811-2-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants. Results: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress. Conclusion: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.

引用

页数：13

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