共 23 条
A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species
被引:302
作者:

Allander, T
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机构: NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA

Emerson, SU
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机构: NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA

Engle, RE
论文数: 0 引用数: 0
h-index: 0
机构: NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA

Purcell, RH
论文数: 0 引用数: 0
h-index: 0
机构: NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA

Bukh, J
论文数: 0 引用数: 0
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机构: NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA
机构:
[1] NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA
[2] NIAID, Infect Dis Lab, Mol Hepatitis Sect, NIH, Bethesda, MD 20892 USA
来源:
关键词:
D O I:
10.1073/pnas.211424698
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Identification of previously unrecognized viral agents in serum or plasma samples is of great medical interest but remains a major challenge, primarily because of abundant host DNA. The current methods, library screening or representational difference analysis (RDA), are very laborious and require selected sample sets. We have developed a simple and reproducible method for discovering viruses in single serum samples that is based on DNase treatment of the serum followed by restriction enzyme digestion and sequence-independent single primer amplification (SISPA) of the fragments, and have evaluated its performance on known viruses. Both DNA viruses and RNA viruses at a concentration of approximate to 10(6) genome equivalents per ml were reproducibly identified in 50 mul of serum. While evaluating the method, two previously unknown parvoviruses were discovered in the bovine sera used as diluent. The near complete genome sequence of each virus was determined; their classification as two species (provisionally named bovine parvoviruses 2 and 3) was confirmed by phylogenetic analysis. Both viruses were found to be frequent contaminants of commercial bovine serum. DNase treatment of serum samples may prove to be a very useful tool for virus discovery. The DNase-SISPA method is suitable for screening of a large number of samples and also enables rapid sequence determination of high-titer viruses.
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页码:11609 / 11614
页数:6
相关论文
共 23 条
[1]
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
[J].
Altschul, SF
;
Madden, TL
;
Schaffer, AA
;
Zhang, JH
;
Zhang, Z
;
Miller, W
;
Lipman, DJ
.
NUCLEIC ACIDS RESEARCH,
1997, 25 (17)
:3389-3402

Altschul, SF
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Madden, TL
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Schaffer, AA
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Zhang, JH
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Zhang, Z
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Miller, W
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA

Lipman, DJ
论文数: 0 引用数: 0
h-index: 0
机构: NHGRI, LAB GENET DIS RES, NIH, BETHESDA, MD 20892 USA
[2]
Serum amyloid P component controls chromatin degradation and prevents antinuclear autoimmunity
[J].
Bickerstaff, MCM
;
Botto, M
;
Hutchinson, WL
;
Herbert, J
;
Tennent, GA
;
Bybee, A
;
Mitchell, DA
;
Cook, HT
;
Butler, PJG
;
Walport, MJ
;
Pepys, MB
.
NATURE MEDICINE,
1999, 5 (06)
:694-697

Bickerstaff, MCM
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Botto, M
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Hutchinson, WL
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Herbert, J
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Tennent, GA
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Bybee, A
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Mitchell, DA
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Cook, HT
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Butler, PJG
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Walport, MJ
论文数: 0 引用数: 0
h-index: 0
机构: Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England

Pepys, MB
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England Imperial Coll Sch Med, Hammersmith Hosp, Immunol Med Unit, London W12 0NN, England
[3]
Toward a surrogate model for hepatitis C virus: An infectious molecular clone of the GB virus-B hepatitis agent
[J].
Bukh, J
;
Apgar, CL
;
Yanagi, M
.
VIROLOGY,
1999, 262 (02)
:470-478

Bukh, J
论文数: 0 引用数: 0
h-index: 0
机构:
NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

Apgar, CL
论文数: 0 引用数: 0
h-index: 0
机构:
NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

Yanagi, M
论文数: 0 引用数: 0
h-index: 0
机构:
NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA NIAID, Hepatitis Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
[4]
COMPLETE NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF BOVINE PARVOVIRUS
[J].
CHEN, KC
;
SHULL, BC
;
MOSES, EA
;
LEDERMAN, M
;
STOUT, ER
;
BATES, RC
.
JOURNAL OF VIROLOGY,
1986, 60 (03)
:1085-1097

CHEN, KC
论文数: 0 引用数: 0
h-index: 0

SHULL, BC
论文数: 0 引用数: 0
h-index: 0

MOSES, EA
论文数: 0 引用数: 0
h-index: 0

LEDERMAN, M
论文数: 0 引用数: 0
h-index: 0

STOUT, ER
论文数: 0 引用数: 0
h-index: 0

BATES, RC
论文数: 0 引用数: 0
h-index: 0
[5]
ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME
[J].
CHOO, QL
;
KUO, G
;
WEINER, AJ
;
OVERBY, LR
;
BRADLEY, DW
;
HOUGHTON, M
.
SCIENCE,
1989, 244 (4902)
:359-362

CHOO, QL
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333

KUO, G
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333

WEINER, AJ
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333

OVERBY, LR
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333

BRADLEY, DW
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333

HOUGHTON, M
论文数: 0 引用数: 0
h-index: 0
机构:
CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333 CTR DIS CONTROL,HEPATITIS BRANCH,ATLANTA,GA 30333
[6]
Contamination and sensitivity issues with a real-time universal 16S rRNA PCR
[J].
Corless, CE
;
Guiver, M
;
Borrow, R
;
Edwards-Jones, V
;
Kaczmarski, EB
;
Fox, AJ
.
JOURNAL OF CLINICAL MICROBIOLOGY,
2000, 38 (05)
:1747-1752

Corless, CE
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England

Guiver, M
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England

Borrow, R
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England

Edwards-Jones, V
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England

Kaczmarski, EB
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England

Fox, AJ
论文数: 0 引用数: 0
h-index: 0
机构: Withington Hosp, Manchester Publ Hlth Lab, Meningococcal Reference Unit, Manchester M20 2LR, Lancs, England
[7]
A RANDOM-PCR METHOD (RPCR) TO CONSTRUCT WHOLE CDNA LIBRARY FROM LOW AMOUNTS OF RNA
[J].
FROUSSARD, P
.
NUCLEIC ACIDS RESEARCH,
1992, 20 (11)
:2900-2900

FROUSSARD, P
论文数: 0 引用数: 0
h-index: 0
[8]
Molecular cloning and disease association of hepatitis G virus: A transfusion-transmissible agent
[J].
Linnen, J
;
Wages, J
;
ZhangKeck, ZY
;
Fry, KE
;
Krawczynski, KZ
;
Alter, H
;
Koonin, E
;
Gallagher, M
;
Alter, M
;
Hadziyannis, S
;
Karayiannis, P
;
Fung, K
;
Nakatsuji, Y
;
Shih, JWK
;
Young, L
;
Piatak, M
;
Hoover, C
;
Fernandez, J
;
Chen, S
;
Zou, JC
;
Morris, T
;
Hyams, KC
;
Ismay, S
;
Lifson, JD
;
Hess, G
;
Foung, SKH
;
Thomas, H
;
Bradley, D
;
Margolis, H
;
Kim, JP
.
SCIENCE,
1996, 271 (5248)
:505-508

Linnen, J
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Wages, J
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

ZhangKeck, ZY
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Fry, KE
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Krawczynski, KZ
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Alter, H
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Koonin, E
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Gallagher, M
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Alter, M
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Hadziyannis, S
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Karayiannis, P
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Fung, K
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Nakatsuji, Y
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Shih, JWK
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Young, L
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Piatak, M
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Hoover, C
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Fernandez, J
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Chen, S
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Zou, JC
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Morris, T
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Hyams, KC
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Ismay, S
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Lifson, JD
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Hess, G
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Foung, SKH
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Thomas, H
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Bradley, D
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Margolis, H
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063

Kim, JP
论文数: 0 引用数: 0
h-index: 0
机构: GENELABS TECHNOL,REDWOOD CITY,CA 94063
[9]
CLONING THE DIFFERENCES BETWEEN 2 COMPLEX GENOMES
[J].
LISITSYN, N
;
LISITSYN, N
;
WIGLER, M
.
SCIENCE,
1993, 259 (5097)
:946-951

LISITSYN, N
论文数: 0 引用数: 0
h-index: 0
机构:
RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA

LISITSYN, N
论文数: 0 引用数: 0
h-index: 0
机构:
RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA

WIGLER, M
论文数: 0 引用数: 0
h-index: 0
机构:
RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA
[10]
Evolutionary relationships among parvoviruses: Virus-host coevolution among autonomous primate parvoviruses and links between adeno-associated and avian parvoviruses
[J].
Lukashov, VV
;
Goudsmit, J
.
JOURNAL OF VIROLOGY,
2001, 75 (06)
:2729-2740

Lukashov, VV
论文数: 0 引用数: 0
h-index: 0
机构: Univ Amsterdam, Acad Med Ctr, Dept Human Retrovirol, NL-1105 AZ Amsterdam, Netherlands

Goudsmit, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Amsterdam, Acad Med Ctr, Dept Human Retrovirol, NL-1105 AZ Amsterdam, Netherlands