Determination of Malachite Green Residues in Fish Using a Highly Sensitive Electrochemiluminescence Method Combined with Molecularly Imprinted Solid Phase Extraction

被引：89

作者：

Guo, Zhiyong ^{[1
]}

Gai, Panpan ^{[1
]}

Hao, Tingting ^{[1
]}

Duan, Jing ^{[1
]}

Wang, Sui ^{[1
]}

机构：

[1] Ningbo Univ, Fac Mat Sci & Chem Engn, State Key Lab Base Novel Funct Mat & Preparat Sci, Ningbo 315211, Zhejiang, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 10期

基金：

中国国家自然科学基金;

关键词：

electrochemiluminescence (ECL); molecularly imprinted solid phase extraction (MISPE); malachite green; leucomalachite green; luminol; fish; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; LEUCOMALACHITE GREEN; GENTIAN-VIOLET; FLOW-INJECTION; AQUATIC PRODUCTS; FLUORESCENCE DETECTION; HPLC DETERMINATION; ALKALINE-SOLUTION; LUMINOL;

D O I：

10.1021/jf2008502

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An electrochemiluminescence (ECL) inhibition method combined with molecularly imprinted solid phase extraction (MISPE) was developed for quantitative determination of malachite green (MG) residues in fish. It was found that MG could strongly inhibit the ECL signal of luminol. Under the optimized conditions, the quenched ECL intensity versus the logarithm of the concentration of MG was in good linear relationship over a concentration range from 20 to 5000 ppt. The method detection limit was found to be about 6 ppt. Molecularly imprinted polymers (MIPs) were synthesized as solid phase extraction (SPE) sorbents, and MISPE was used for the selective extraction and purification of MG. By carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which could convert leucomalachite green (LMG) into MG, this method was successfully applied to determine MG residues in fish. A possible mechanism for the quenching effects of MG on luminol was also proposed.

引用

页码：5257 / 5262

页数：6

共 36 条

[1] Multiresidue method for the triphenylmethane dyes in fish: Malachite green, crystal (gentian) violet, and brilliant green [J].

Andersen, Wendy C. ;

Turnipseed, Sherri B. ;

Karbiwnyk, Christine M. ;

Lee, Rebecca H. ;

Clark, Susan B. ;

Rowe, W. Douglas ;

Madson, Mark R. ;

Miller, Keith E. .

ANALYTICA CHIMICA ACTA, 2009, 637 (1-2) :279-289

[2] Sonolytic, photocatalytic and sonophotocatalytic degradation of malachite green in aqueous solutions [J].

Berberidou, C. ;

Poulios, I. ;

Xekoukoulotakis, N. P. ;

Mantzavinos, D. .

APPLIED CATALYSIS B-ENVIRONMENTAL, 2007, 74 (1-2) :63-72

[3] Determination of residues of malachite green in aquatic animals [J].

Bergwerff, AA ;

Scherpenisse, P .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 788 (02) :351-359

[4] HPLC Determination and MS Confirmation of Malachite Green, Gentian Violet, and Their Leuco Metabolite Residues in Channel Catfish Muscle [J].

Chen, Guoying ;

Miao, Shui .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (12) :7109-7114

[5] Potential-dependent electrochemiluminescence of luminol in alkaline solution at a gold electrode [J].

Cui, H ;

Zhang, ZF ;

Zou, GZ ;

Lin, XQ .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2004, 566 (02) :305-313

[6] Electrochemiluminescence of luminol in alkaline solution at a paraffin-impregnated graphite electrode [J].

Cui, H ;

Zou, GZ ;

Lin, XQ .

ANALYTICAL CHEMISTRY, 2003, 75 (02) :324-331

[7] An electrochemiluminescent biosensor for vitamin C based on inhibition of luminol electrochemiluminescence on graphite/poly(methyl methacrylate) composite electrode [J].

Dai, Hong ;

Wu, Xiaoping ;

Wang, Youmei ;

Zhou, Weichao ;

Chen, Guonan .

ELECTROCHIMICA ACTA, 2008, 53 (16) :5113-5117

[8] Confirmatory analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in salmon by liquid chromatography-tandem mass spectrometry [J].

Dowling, Geraldine ;

Mulder, Patrick P. J. ;

Duffy, Conor ;

Regan, Liam ;

Smyth, Malcolm R. .

ANALYTICA CHIMICA ACTA, 2007, 586 (1-2) :411-419

[9] Tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence [J].

Gorman, BA ;

Francis, PS ;

Barnett, NW .

ANALYST, 2006, 131 (05) :616-639

[10] Liquid chromatography with time-of-flight mass spectrometry for simultaneous determination of chemotherapeutant residues in salmon [J].

Hernando, MD ;

Mezcua, M ;

Suárez-Barcena, JM ;

Fernández-Alba, AR .

ANALYTICA CHIMICA ACTA, 2006, 562 (02) :176-184

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