The effectiveness of colostral antibodies for preventing bovine leukemia virus (BLV) infection in vitro

被引：19

作者：

Konishi, Misako ^{[1
]}

Ishizaki, Hiroshi ^{[2
]}

Kameyama, Ken-ichiro ^{[3
]}

Murakami, Kenji ^{[4
]}

Yamamoto, Takehisa ^{[1
]}

机构：

[1] Natl Inst Anim Hlth, Div Viral Dis & Epidemiol, Epidemiol Unit, NARO 3-1-5 Kannondai, Tsukuba, Ibaraki 3050856, Japan

[2] Inst Livestock & Grassland Sci, Grazing Anim Unit, Div Grassland Farming, NARO 768 Senbonmatsu, Nasushiobara, Tochigi 3292793, Japan

[3] NARO, Natl Inst Anim Hlth, Exot Dis Res Stn, Josuihoncho, Tokyo 1870022, Japan

[4] Iwate Univ, Fac Agr, Cooperat Dept Vet Med, 3-18-8 Ueda, Morioka, Iwate 0208550, Japan

来源：

BMC VETERINARY RESEARCH | 2018年 / 14卷

基金：

日本学术振兴会;

关键词：

Bovine leukemia virus; Colostral antibody; Proviral load; Prevention; LEUKOSIS VIRUS; DAIRY; MILK; TRANSMISSION; HEMATOLOGY; COMPLEMENT; CATTLE; PROTECTION; CELLS; CALF;

D O I：

10.1186/s12917-018-1724-5

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

BackgroundBovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). The incidence of EBL in Japan is increasing annually; and the cases of EBL in cattle younger than 2years old has been reported. Therefore, it is vital to find a method to control BLV infection, especially in young calves. In this study, to evaluate the protective ability of colostral antibodies against BLV infection, as well as the potential for BLV infection mediated by colostrum/milk, we investigated temporal fluctuations in the anti-BLV antibody titer and BLV proviral load (PVL) in colostrum/milk and peripheral blood of six infected dams during lactation. The association between PVL and antibody titer in colostrum and peripheral blood was then investigated using samples from a further twenty-seven cattle. Antibody concentrations were measured with a Syncytium-induction Inhibition Assay using colostral/milk whey and serum. PVL in peripheral blood and colostrum was measured by real-time PCR.ResultsColostral antibodies showed high inhibitory activity until day 3 of lactation. The antibody titer and PVL in peripheral blood showed lesser changes than those in colostrum/milk throughout lactation. The colostral antibody titer was significantly higher than the serum antibody titer in all samples, whereas the colostrum PVL was significantly lower than the blood PVL. The blood PVL showed a significant correlation with serum antibody titer, colostrum PVL, and colostral antibody titer. However, there were no major correlations between the serum and colostral antibody titers.ConclusionsThis is the first report investigating the temporal changes in colostral antibody titer in terms of inhibiting BLV infection in vitro. The results of antibody detection by Syncytium-induction Inhibition Assay suggested that the protective activity of the colostral antibodies against BLV infection would be conferred by anti-BLV gp51 antibody. The high antibody titer of colostral whey suggests that colostral whey could be a potential source of antibodies with a low risk of infection in neonatal calves.

引用

页数：9