Nuclease Tudor-SN Is Involved in Tick dsRNA-Mediated RNA Interference and Feeding but Not in Defense against Flaviviral or Anaplasma phagocytophilum Rickettsial Infection

被引：10

作者：

Ayllon, Nieves ^{[1
]}

Naranjo, Victoria ^{[1
,2
]}

Hajdusek, Ondrej ^{[3
]}

Villar, Margarita ^{[1
]}

Galindo, Ruth C. ^{[1
,2
]}

Kocan, Katherine M. ^{[2
]}

Alberdi, Pilar ^{[1
]}

Sima, Radek ^{[3
]}

Cabezas-Cruz, Alejandro ^{[1
,4
]}

Rueckert, Claudia ^{[5
]}

Bell-Sakyi, Lesley ^{[5
]}

Kazimirova, Mÿria ^{[6
]}

Havlikova, Sabna ^{[7
]}

Klempa, Boris ^{[7
]}

Kopacek, Petr ^{[3
]}

de la Fuente, Jose ^{[1
,2
]}

机构：

[1] CSIC UCLM JCCM, Inst Invest Recursos Cineget IREC, SaBio, Ciudad Real 13005, Spain

[2] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA

[3] Acad Sci Czech Republ, Inst Parasitol, Ctr Biol, CR-37005 Ceske Budejovice, Czech Republic

[4] Univ Lille Nord France, CNRS, Inst Pasteur Lille, CIIL,INSERM U1019,UMR 8204, Lille, France

[5] Pirbright Inst, Woking GU24 0NF, Surrey, England

[6] Slovak Acad Sci, Inst Zool, Bratislava 84506, Slovakia

[7] Slovak Acad Sci, Inst Virol, Bratislava 84505, Slovakia

来源：

PLOS ONE | 2015年 / 10卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

QUANTITATIVE PROTEOMICS; GENE-EXPRESSION; CELL-LINES; MACHINERY; INSIGHTS; ACCURATE; DISEASE; BINDING; GROWTH;

D O I：

10.1371/journal.pone.0133038

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tudor staphylococcal nuclease (Tudor-SN) and Argonaute (Ago) are conserved components of the basic RNA interference (RNAi) machinery with a variety of functions including immune response and gene regulation. The RNAi machinery has been characterized in tick vectors of human and animal diseases but information is not available on the role of Tudor-SN in tick RNAi and other cellular processes. Our hypothesis is that tick Tudor-SN is part of the RNAi machinery and may be involved in innate immune response and other cellular processes. To address this hypothesis, Ixodes scapularis and I. ricinus ticks and/or cell lines were used to annotate and characterize the role of Tudor-SN in dsRNA-mediated RNAi, immune response to infection with the rickettsia Anaplasma phagocytophilum and the flaviviruses TBEV or LGTV and tick feeding. The results showed that Tudor-SN is conserved in ticks and involved in dsRNA-mediated RNAi and tick feeding but not in defense against infection with the examined viral and rickettsial pathogens. The effect of Tudor-SN gene knockdown on tick feeding could be due to down-regulation of genes that are required for protein processing and blood digestion through a mechanism that may involve selective degradation of dsRNAs enriched in G:U pairs that form as a result of adenosine-to-inosine RNA editing. These results demonstrated that Tudor-SN plays a role in tick RNAi pathway and feeding but no strong evidence for a role in innate immune responses to pathogen infection was found.

引用

页数：18

共 51 条

[1] RNA interference inhibits replication of tick-borne encephalitis virus in vitro [J].

Achazi, Katharina ;

Patel, Pranav ;

Paliwal, Ravish ;

Radonic, Aleksandar ;

Niedrig, Matthias ;

Donoso-Mantke, Oliver .

ANTIVIRAL RESEARCH, 2012, 93 (01) :94-100

[2] RNA interference: applicability in tick research [J].

Aljamali, MN ;

Sauer, JR ;

Essenberg, RC .

EXPERIMENTAL AND APPLIED ACAROLOGY, 2002, 28 (01) :89-96

[3] RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease [J].

Alsford, Sam ;

Kemp, Louise E. ;

Kawahara, Taemi ;

Horn, David .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2010, 174 (01) :70-73

[4] Approximate likelihood-ratio test for branches: A fast, accurate, and powerful alternative [J].

Anisimova, Maria ;

Gascuel, Olivier .

SYSTEMATIC BIOLOGY, 2006, 55 (04) :539-552

[5]

[Anonymous], 2005, PHYLIP (phylogeny inference package) version 3.6

[6] Scavenger Receptor Mediates Systemic RNA Interference in Ticks [J].

Aung, Kyaw Min ;

Boldbaatar, Damdinsuren ;

Umemiya-Shirafuji, Rika ;

Liao, Min ;

Xuan Xuenan ;

Suzuki, Hiroshi ;

Galay, Remil Linggatong ;

Tanaka, Tetsuya ;

Fujisaki, Kozo .

PLOS ONE, 2011, 6 (12)

[7] Systems Biology of Tissue-Specific Response to Anaplasma phagocytophilum Reveals Differentiated Apoptosis in the Tick Vector Ixodes scapularis [J].

Ayllon, Nieves ;

Villar, Margarita ;

Galindo, Ruth C. ;

Kocan, Katherine M. ;

Sima, Radek ;

Lopez, Juan A. ;

Vazquez, Jesus ;

Alberdi, Pilar ;

Cabezas-Cruz, Alejandro ;

Kopacek, Petr ;

de la Fuente, Jose .

PLOS GENETICS, 2015, 11 (03)

[8] Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells [J].

Ayllon, Nieves ;

Villar, Margarita ;

Busby, Ann T. ;

Kocan, Katherine M. ;

Blouin, Edmour F. ;

Bonzon-Kulichenko, Elena ;

Galindo, Ruth C. ;

Mangold, Atilio J. ;

Alberdi, Pilar ;

Perez de la Lastra, Jose M. ;

Vazquez, Jesus ;

de la Fuente, Jose .

INFECTION AND IMMUNITY, 2013, 81 (07) :2415-2425

[9] RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface [J].

Barnard, Annette-Christi ;

Nijhof, Ard M. ;

Fick, Wilma ;

Stutzer, Christian ;

Maritz-Olivier, Christine .

GENES, 2012, 3 (04) :702-741

[10] Gene silencing in tick cell lines using small interfering or long double-stranded RNA [J].

Barry, Gerald ;

Alberdi, Pilar ;

Schnettler, Esther ;

Weisheit, Sabine ;

Kohl, Alain ;

Fazakerley, John K. ;

Bell-Sakyi, Lesley .

EXPERIMENTAL AND APPLIED ACAROLOGY, 2013, 59 (03) :319-338

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