A comparison between slit densitometry and video densitometry for quantitation in thin layer chromatography

被引：12
作者：
Mustoe, SP [1 ]
McCrossen, D [1 ]
机构：
[1] GlaxoSmithKline, Tonbridge TN11 9AN, England
来源：
CHROMATOGRAPHIA | 2001年 / 53卷 / Suppl 1期
关键词：
thin layer chromatography; slit densitometry; video densitometry; quantitation;
D O I：
10.1007/BF02490381
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The work presented here compares the performance of slit with video densitometry Linearity, precision and detection limits were studied for some test dyes. Slit densitometry was performed using commercial TLC apparatus and video densitometry using a conventional flat-bed scanner with commercial software. Results show that video densitometry has equivalent precision compared with slit densitometry, however, it has a broader linear range and better sensitivity for the compounds studied. Other attributes of video densitometry are highlighted.
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收藏
页码：S474 / S477
页数：4
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