Enzyme Immobilization on Synthesized Nanoporous Silica Particles and their Application in a Bi-enzymatic Reaction

被引：23

作者：

Engelmann, Claudia ^{[1
]}

Ekambaram, Narendhiran ^{[1
]}

Johannsen, Jens ^{[2
]}

Fellechner, Oliver ^{[3
]}

Waluga, Thomas ^{[2
]}

Fieg, Georg ^{[2
]}

Liese, Andreas ^{[1
]}

Bubenheim, Paul ^{[1
]}

机构：

[1] Hamburg Univ Technol, Inst Tech Biocatalysis, Denickestr 15, D-21073 Hamburg, Germany

[2] Hamburg Univ Technol, Inst Proc & Plant Engn, Schwarzenberg Campus 4, D-21073 Hamburg, Germany

[3] Hamburg Univ Technol, Inst Thermal Separat Proc, Eissendorfer Str 38, D-21073 Hamburg, Germany

来源：

CHEMCATCHEM | 2020年 / 12卷 / 08期

关键词：

Biocatalysis; Cofactor regeneration; Immobilization; Aromatic compounds; Alcohol dehydrogenase; METAL-ORGANIC FRAMEWORKS; ALCOHOL-DEHYDROGENASE; COVALENT BINDING;

D O I：

10.1002/cctc.201902293

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The application of enzymes presents a great advantage regarding highly selective reactions; however, it involves also challenges due to their sensitivity. Immobilization offers one strategy to overcome those challenges enabling enzyme stabilization, as well as retention. In the present study, covalent attachment on hydrophilic amino-functionalized carriers is found to be the most promising immobilization method for the investigated reaction system. To achieve this, a novel method for preparation of silica particles with subsequent amino-functionalization is developed to prepare spherical carriers for enzyme immobilization, whereby high porosities are obtained based on polymerization. With these particles, immobilization of an alcohol dehydrogenase and a formate dehydrogenase is realized with residual activities of 70 and 80 % after 12 consecutive batches, respectively. The two immobilized enzymes are used in the reduction of cinnamyl aldehyde with in situ cofactor regeneration, obtaining a conversion of 100 % and up to 10-fold higher turnover numbers compared to the free enzyme.

引用

页码：2245 / 2252

页数：8

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