A 7.4-Å projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography

被引：8
|
作者：
Boekema, EJ
Stuart, M
Koning, RI
Keegstra, W
Brisson, A
Verheij, HM
Dekker, N
机构：
[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands
[2] Univ Utrecht, Biomembrane Inst, Dept Enzymol & Prot Engn, Ctr Biomembranes & Lipid Enzymol, Utrecht, Netherlands
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 1998年 / 123卷 / 01期
关键词：
D O I：
10.1006/jsbi.1998.4013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Outer membrane phospholipase A (OMPLA) is one of the few enzymes present in the outer membrane of Escherichia coli. Two-dimensional crystals of OMPLA were grown by reconstitution of purified protein into lipid bilayers via detergent dialysis and were studied by electron crystallography. A 7.4-Angstrom projection map reveals OMPLA molecules exhibiting an oval-shaped domain of 30 x 20 Angstrom resembling the beta-barrel structure characteristic of porins, which is associated with a 25-Angstrom elongated domain of lower density. (C) 1998 Academic Press.
引用
收藏
页码：67 / 71
页数：5
相关论文
共 50 条
  • [1] The projection structure of Kch, a putative potassium channel in Escherichia coli, by electron crystallography
    Kuang, Qie
    Purhonen, Pasi
    Jegerschold, Caroline
    Hebert, Hans
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2014, 1838 (01): : 237 - 243
  • [2] Unusual catalytic triad of Escherichia coli outer membrane phospholipase A
    Kingma, RL
    Fragiathaki, M
    Snijder, HJ
    Dijkstra, BW
    Verheij, HM
    Dekker, N
    Egmond, MR
    BIOCHEMISTRY, 2000, 39 (33) : 10017 - 10022
  • [3] Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A
    Dekker, N
    Tommassen, J
    Lustig, A
    Rosenbusch, JP
    Verheij, HM
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (06) : 3179 - 3184
  • [4] Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography
    Ringler, P
    Borgnia, MJ
    Stahlberg, H
    Maloney, PC
    Agre, P
    Engel, A
    JOURNAL OF MOLECULAR BIOLOGY, 1999, 291 (05) : 1181 - 1190
  • [5] Structure and Assembly of Escherichia Coli Outer Membrane Protein A
    Reusch, Rosetta N.
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 473A - 473A
  • [6] Escherichia coli outer membrane phospholipase A: Role of two serines in enzymatic activity
    Brok, RGPM
    Belandia, IU
    Dekker, N
    Tommassen, J
    Verheij, HM
    BIOCHEMISTRY, 1996, 35 (24) : 7787 - 7793
  • [7] Core structure of the outer membrane lipoprotein from Escherichia coli at 1.9 A resolution
    Shu, W
    Liu, J
    Ji, H
    Lu, M
    JOURNAL OF MOLECULAR BIOLOGY, 2000, 299 (04) : 1101 - 1112
  • [8] Crystal structure of the outer membrane active transporter FepA from Escherichia coli
    Buchanan, SK
    Smith, BS
    Venkatramani, L
    Xia, D
    Esser, L
    Palnitkar, M
    Chakraborty, R
    van der Helm, D
    Deisenhofer, J
    NATURE STRUCTURAL BIOLOGY, 1999, 6 (01): : 56 - 63
  • [9] Crystal structure of the outer membrane active transporter FepA from Escherichia coli
    Buchanan, SK
    Smith, BS
    Venkatramani, L
    Xia, D
    Esser, L
    Palnitkar, M
    Chakraborty, R
    Van der Helm, D
    Deisenhofer, J
    JOURNAL OF GENERAL PHYSIOLOGY, 2000, 116 (01): : 1A - 1A
  • [10] Crystal structure of the outer membrane active transporter FepA from Escherichia coli
    Buchanan S.K.
    Smith B.S.
    Venkatramani L.
    Xia D.
    Esser L.
    Palnitkar M.
    Chakraborty R.
    Van Der Helm D.
    Deisenhofer J.
    Nature Structural Biology, 1999, 6 (1) : 56 - 63
12345