Replication of Legionella pneumophila in floating biofilms

被引：23

作者：

Declerck, Priscilla ^{[1
]}

Behets, Jonas ^{[1
]}

van Hoef, Vincent ^{[1
]}

Ollevier, Frans ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Inst Zool, Aquat Ecol Lab, B-3000 Louvain, Belgium

来源：

CURRENT MICROBIOLOGY | 2007年 / 55卷 / 05期

关键词：

floating biofilms; Legionella pneumophila; Acanthamoeba castellanii; Intracellular replication;

D O I：

10.1007/s00284-007-9006-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Biofilms are a major source of human pathogenic Legionella pneumophila in aquatic systems. In this study, we investigated the capacity of L. pneumophila to colonize floating biofilms and the impact of Acanthamoeba castellanii on the replication of biofilm-associated Legionella. Biofilms were grown in Petri dishes and consisted of Aeromonas hydrophila, Escherichia coli, Flavobacterium breve, and Pseudomonas aeruginosa. Six hours following inoculation, Legionella were detected in floating biofilms in mean concentrations of 1.4 x 10(4) cells/cm(2) (real-time polymerase chain reaction) and 8.3 x 10(2) CFU/cm(2) (culture). Two-way analysis of variance tests and fluorescent in situ hybridization clearly proved that increased biofilm-associated L. pneumophila concentrations were the result of intracellular replication in A. castellanii. Forty-eight hours after the introduction of A. castellanii in the Petri dishes, 90 +/- 0.8% of the amoebae (infection rate) were completely filled with highly metabolic active L. pneumophila (mean infection intensity).

引用

页码：435 / 440

页数：6

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