Human mesenchymal stem cells and renal tubular epithelial cells differentially influence monocyte-derived dendritic cell differentiation and maturation

被引:51
作者
Kronsteiner, Barbara [1 ,2 ,3 ]
Peterbauer-Scherb, Anja [1 ,3 ]
Grillari-Voglauer, Regina [4 ]
Redl, Heinz [2 ,3 ]
Gabriel, Christian [1 ,3 ]
van Griensven, Martijn [2 ,3 ]
Wolbank, Susanne [2 ,3 ]
机构
[1] Red Cross Blood Transfus Serv Upper Austria, A-4017 Linz, Austria
[2] AUVA Res Ctr, Ludwig Boltzmann Inst Expt & Clin Traumatol, A-1200 Vienna, Austria
[3] Austrian Cluster Tissue Regenerat, Vienna, Austria
[4] Univ Nat Resources & Appl Life Sci, Inst Appl Microbiol, A-1190 Vienna, Austria
关键词
Mesenchymal stem cells; Renal tubular epithelial cells; Dendritic cells; Immunomodulation; T-CELLS; PROSTAGLANDIN E-2; ANTIGEN PRESENTATION; ADIPOSE-TISSUE; STROMAL CELLS; HLA-G; INHIBIT; ACTIVATION; PROLIFERATION; EXPRESSION;
D O I
10.1016/j.cellimm.2010.11.001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mesenchymal stem cells (MSCs) possess immunosuppressive properties. But also fully differentiated human renal tubular epithelial cells (RTECs) are able to modulate T-cell proliferation in vitro. In this study we compared two MSC populations, human adipose derived stem cells (ASCs) and human amniotic mesenchymal stromal cells (hAMSCs), and RTECs regarding their potential to inhibit monocyte-derived dendritic cell (DC) differentiation and maturation in indirect co-culture. In the presence of hAMSCs and RTECs, monocytes stimulated to undergo DC differentiation were inhibited to acquire surface phenotype of immature and mature DCs. In contrast, ASCs showed only limited suppressive capacity. Secretion of IL-12p70 was suppressed in hAMSC co-cultures and high IL-10 levels were detected in all co-cultures. Prostaglandin E-2 was found in ASC and hAMSC co-cultures, whereas soluble human leukocyte antigen-G was highly elevated only in RTEC co-cultures. Thus, inhibition of DC generation by MSCs and RTECs might be mediated by different soluble factors. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:30 / 38
页数:9
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