Randomized Trial of Glucosamine and Chondroitin Supplementation on Inflammation and Oxidative Stress Biomarkers and Plasma Proteomics Profiles in Healthy Humans

被引:47
作者
Navarro, Sandi L. [1 ]
White, Emily [1 ]
Kantor, Elizabeth D. [2 ]
Zhang, Yuzheng [1 ]
Rho, Junghyun [1 ]
Song, Xiaoling [1 ]
Milne, Ginger L. [3 ]
Lampe, Paul D. [1 ]
Lampe, Johanna W. [1 ]
机构
[1] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA
[2] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA
[3] Vanderbilt Univ, Sch Med, Div Clin Pharmacol, Nashville, TN 37212 USA
来源
PLOS ONE | 2015年 / 10卷 / 02期
基金
美国国家卫生研究院;
关键词
DIETARY-SUPPLEMENTS; KNEE OSTEOARTHRITIS; CANCER; PROTEIN; DNA; RECOMMENDATIONS; MICROTUBULES; ASSOCIATIONS; MECHANISMS; EXPRESSION;
D O I
10.1371/journal.pone.0117534
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background Glucosamine and chondroitin are popular non-vitamin dietary supplements used for osteoarthritis. Long-term use is associated with lower incidence of colorectal and lung cancers and with lower mortality; however, the mechanism underlying these observations is unknown. In vitro and animal studies show that glucosamine and chondroitin inhibit NF-kB, a central mediator of inflammation, but no definitive trials have been done in healthy humans. Methods We conducted a randomized, double-blind, placebo-controlled, cross-over study to assess the effects of glucosamine hydrochloride (1500 mg/d) plus chondroitin sulfate (1200 mg/d) for 28 days compared to placebo in 18 (9 men, 9 women) healthy, overweight (body mass index 25.0-32.5 kg/m(2)) adults, aged 20-55 y. We examined 4 serum inflammatory biomarkers: C-reactive protein (CRP), interleukin 6, and soluble tumor necrosis factor receptors I and II; a urinary inflammation biomarker: prostaglandin E2-metabolite; and a urinary oxidative stress biomarker: F-2-isoprostane. Plasma proteomics on an antibody array was performed to explore other pathways modulated by glucosamine and chondroitin. Results Serum CRP concentrations were 23% lower after glucosamine and chondroitin compared to placebo (P = 0.048). There were no significant differences in other biomarkers. In the proteomics analyses, several pathways were significantly different between the interventionsafter Bonferroni correction, the most significant being a reduction in the "cytokine activity" pathway (P = 2.6 x 10(-16)), after glucosamine and chondroitin compared to placebo. Conclusion Glucosamine and chondroitin supplementation may lower systemic inflammation and alter other pathways in healthy, overweight individuals. This study adds evidence for potential mechanisms supporting epidemiologic findings that glucosamine and chondroitin are associated with reduced risk of lung and colorectal cancer.
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页数:20
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