Countercurrent chromatographic purification of polysaccharides from Achyranthes bidentata with an aqueous two-phase system using a cross-axis coil planet centrifuge

被引：17

作者：

Chao, ZM

Shibusawa, Y

Shindo, H

Ito, Y

机构：

[1] China Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[2] Tokyo Univ Pharm & Life Sci, Dept Analyt Chem, Sch Pharm, Tokyo, Japan

[3] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 12期

关键词：

countercurrent chromatography; cross-axis coil planet centrifuge; polysaccharides; partition coefficient;

D O I：

10.1081/JLC-120021758

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross-axis coil planet centrifuge (X-axis CPC). The purification was performed with an aqueous polymer two-phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000, 8% (w/w) potassium dihydrogen phosphate, and 8% (w/w) dipotassium hydrogen phosphate buffer at pH 6.8. The preparative separation of the crude sample (2 g) was successfully performed, yielding 1.1 g of polysaccharides at high purity.

引用

页码：1895 / 1903

页数：9

共 7 条

[1] IMPROVED CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE FOR PERFORMING COUNTERCURRENT CHROMATOGRAPHY .2. STUDIES ON RETENTION OF STATIONARY PHASE IN SHORT COILS AND PREPARATIVE SEPARATIONS IN MULTILAYER COILS [J].