The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR

被引：92

作者：

Konnai, S ^{[1
]}

Usui, T ^{[1
]}

Ohashi, K ^{[1
]}

Onuma, M ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Sapporo, Hokkaido 0600818, Japan

来源：

VETERINARY MICROBIOLOGY | 2003年 / 94卷 / 04期

关键词：

real-time RT-PCR; SYBR Green I; bovine cytokine; ovine cytokine; bovine leukemia virus;

D O I：

10.1016/S0378-1135(03)00119-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

For a practical need, fast and efficient methods to quantify mRNA expression are expecting. By using real-time reverse transcription polymerase chain reaction (RT-PCR) with the double-stranded DNA-binding dye SYBR Green I as a novel method, cytokine profiles (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12p40 and IFN-gamma) were analyzed in peripheral blood mononuclear cells (PBMCs) from bovine leukemia virus (BLV)-infected animals. In aleukemic cattle, IFN-gamma and IL-12p40 mRNA expression was significantly increased compared to those in cattle with persistent lymphocytosis. The similar results were obtained in the case of sheep experimentally infected with BLV. Real-time quantitative PCR technique is an applicable technique for analysis of cytokine profiles in field. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：283 / 294

页数：12

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