Ecotoxicological evaluation of zebrafish liver (Danio rerio) induced by dibutyl phthalate

被引：76

作者：

Song, Peipei ^{[1
]}

Jiang, Nan ^{[1
]}

Zhang, Kaiqu ^{[1
]}

Li, Xianxu ^{[1
]}

Li, Na ^{[1
]}

Zhang, Youai ^{[1
]}

Wang, Qian ^{[1
]}

Wang, Jun ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Resources & Environm, Key Lab Agr Environm, Tai An 271018, Shandong, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2022年 / 425卷

基金：

中国国家自然科学基金;

关键词：

Dibutyl phthalate (DBP); Zebrafish; Oxidative and DNA damage; Neurotoxicity; Antioxidant and apoptosis genes; OXIDATIVE STRESS RESPONSES; ACETYLCHOLINESTERASE ACTIVITY; ACHE ACTIVITY; DNA-DAMAGE; APOPTOSIS; EXPOSURE; EXPRESSION; TOXICITY; DEFENSE; NEUROTOXICITY;

D O I：

10.1016/j.jhazmat.2021.128027

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Dibutyl phthalate (DBP), one of the most commonly applied plasticizers, has been frequently detected in the aquatic environment, posing potential risks to aquatic organisms. Currently, reports about the toxicity of zebrafish liver with DBP exposure are rare, and the toxic mechanism is still not clear. In this study, zebrafish (Danio rerio) were used to explore the ecotoxicological effects of DBP from the physiological, biochemical, genetic, and molecular levels. The results showed oxidative stress, lipid peroxidation, and DNA damage occurred in zebrafish liver according to changes in antioxidant enzymes, MDA and 8-OHdG content. AchE activity was always active, and negatively correlated with the DBP concentration. The expression of Cu/Zn-sod and gpx genes were similar to that of antioxidant enzymes from 7 to 21 days, while in the end, the inconsistent result appeared due to the time lag effect in protein modification, gene transcription and translation. Besides, the mRNA abundance of Caspase-3 and p53 were upregulated, showing a "dose-response relationship. The integrated biomarker reaction indicated that the effects of exposure time on zebrafish liver was 14th day> 28th day> 7th day> 21th day. These results are of great significance to evaluate the toxicological effects and explore the toxic mechanism of DBP on aquatic organisms.

引用

页数：9

共 61 条

[1] Suppressive effects of long-term exposure to P-nitrophenol on gonadal development, hormonal profile with disruption of tissue integrity, and activation of caspase-3 in male Japanese quail (Coturnix japonica) [J].