Polymyxin B Resistance and Biofilm Formation in Vibrio cholerae Are Controlled by the Response Regulator CarR

被引：50
作者：
Bilecen, Kivanc [1 ]
Fong, Jiunn C. N. [1 ]
Cheng, Andrew [1 ]
Jones, Christopher J. [1 ]
Zamorano-Sanchez, David [1 ]
Yildiz, Fitnat H. [1 ]
机构：
[1] Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA
来源：
INFECTION AND IMMUNITY | 2015年 / 83卷 / 03期
关键词：
ANTIMICROBIAL PEPTIDES ACTIVATE; RUGOSE COLONY MORPHOLOGY; VPS BIOSYNTHESIS GENES; O1; EL-TOR; EXPRESSION; SYSTEM; IDENTIFICATION; QUANTIFICATION; COLONIZATION; PHENOTYPES;
D O I：
10.1128/IAI.02700-14
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Two-component systems play important roles in the physiology of many bacterial pathogens. Vibrio cholerae's CarRS two-component regulatory system negatively regulates expression of vps (Vibrio polysaccharide) genes and biofilm formation. In this study, we report that CarR confers polymyxin B resistance by positively regulating expression of the almEFG genes, whose products are required for glycine and diglycine modification of lipid A. We determined that CarR directly binds to the regulatory region of the almEFG operon. Similarly to a carR mutant, strains lacking almE, almF, and almG exhibited enhanced polymyxin B sensitivity. We also observed that strains lacking almE or the almEFG operon have enhanced biofilm formation. Our results reveal that CarR regulates biofilm formation and antimicrobial peptide resistance in V. cholerae.
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收藏
页码：1199 / 1209
页数：11
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