Whole-Genome Expression Analysis of Mycobacterium leprae and Its Clinical Application

被引：0

作者：

Akama, Takeshi ^{[1
]}

Suzuki, Koichi ^{[1
]}

Tanigawa, Kazunari ^{[1
]}

Nakamura, Kazuaki ^{[1
]}

Kawashima, Akira ^{[1
]}

Wu, Huhehasi ^{[1
]}

Sue, Mariko ^{[1
]}

Yoshihara, Aya ^{[1
]}

Ishido, Yuko ^{[1
]}

Ishii, Norihisa ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Leprosy Res Ctr, Higashimurayama, Tokyo 1890002, Japan

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2010年 / 63卷 / 06期

关键词：

GENE-EXPRESSION; ESCHERICHIA-COLI; NONCODING RNAS; PROTEIN; PSEUDOGENES; SEQUENCE; IDENTIFICATION; LEPROSY; ARRAY; TRANSCRIPTOME;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The whole-genome sequence analysis of Mycobacterium leprae, which was completed in 2001, revealed the characteristics of this microbe's genomic structure Half of the M leprae genome consists of a limited number of protein-coding genes and the rest comprises non-coding regions and pseudogenes We performed membrane array and tiling array analyses to analyze the gene-expression profile of the M leprae genome and found that pseudogenes and non-coding regions were expressed similarly to coding regions at the RNA level The RNA expressions were confirmed by real-time PCR analysis Expression of these RNAs in clinical samples showed varying patterns among patients, thus indicating that the analysis of RNA expression patterns, including non-coding regions and pseudogenes, may be useful for understanding the pathological state, prognosis, and assessment of therapeutic progress in leprosy

引用

页码：387 / 392

页数：6

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