Cloning and expression of human UDP-glucuronosyltransferase (UGT) 1A8

The mRNA expression of human UDP-glucuronosyltransferase 1A8 (UGT1A8) has been found in jejunum, ileum, and colon but not in liver. A cDNA with a complete UGT1A8 coding region was amplified from total human ileal RNA by reverse transcriptase-polymerase chain reaction and inserted into the mammalian expression vector, pcDNA3. Lysates of HK293 cells expressing UGT1A8 revealed the expression of a protein with a molecular mass of 56 kDa by Western blot analysis. Transiently expressed human UGT1A8 shows glucuronidation activities with coumarins, anthraquinones, flavonoids, phenolic compounds, catechol estrogens, 17-hydroxyandrogens, primary amines such as the carcinogen 4-aminobiphenyl, and certain opioids. This UGT may play an important role in the detoxification of xenobiotics in human intestine. (C) 1998 Academic Press.