Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas

被引:123
作者
Luo, Bing [1 ]
Wang, Yun [1 ]
Wang, Xiao-Feng [1 ]
Liang, Hua [1 ]
Yan, Li-Ping [1 ]
Huang, Bao-Hua [2 ]
Zhao, Peng [3 ]
机构
[1] Qingdao Univ, Coll Med, Dept Microbiol, Qingdao 266021, Shandong Provin, Peoples R China
[2] Yantai Yuhuangding Hosp, Dept Oncol, Yantai 264002, Shandong, Peoples R China
[3] Qingdao Univ, Coll Med, Affiliated Hosp, Dept Pathol, Qingdao 266003, Shandong Provin, Peoples R China
基金
中国国家自然科学基金;
关键词
Gastric carcinomas; Epstein-Barr virus; Gene expression;
D O I
10.3748/wjg.v11.i5.629
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To understand the expression of latent and lytic genes of Epstein-Barr virus (EBV) in EBV-associated gastric carcinoma (EBVaGC) and to explore the relationship between EBV-encoded genes and development of EBVaGC at molecular level. METHODS: One hundred and seventy-two gastric carcinoma tissues and 172 corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-Southern blotting. EBV-encoded small RNA (EBER) 1 of the PCR positive specimens was detected by in situ hybridization (ISH). Gastric carcinomas with positive EBER1 signals were classified as EBVaGCs. RT-PCR and Southern hybridization were applied to the detection of expression of nuclear antigen (EBNA) promoters (Qp, Wp and Cp), EBNA 1 and EBNA 2, latent membrane proteins (LMP) 1, 2A and 2B and lytic genes (immediate early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBVaGCs. RESULTS: Eleven EBV positive samples existed in gastric carcinoma tissues (6.39%). No EBV positive sample was found in corresponding para-carcinoma tissues. The difference between EBV positivity in carcinoma tissues and corresponding para-carcinoma tissues was significant (chi(2) = 9.0909, P = 0.0026). Transcripts of Qp and EBNA1 were detected in all the 11 EBVaGCs, while both Wp and Cp were silent. EBNA2, LMP1 and LMP2B mRNA were absent in all the cases, while LMP2A mRNA was detected in 4 of the 11 cases. Of the 11 EBVaGCs, 7 exhibited BcLF1 transcripts and 2 exhibited BHRF1 transcripts. The transcripts of BZLF1 and BARF1 were detected in 5 cases, respectively. No BLLF1 and BRLF mRNA were detected. CONCLUSION: The latent pattern of EBV in gastric carcinoma corresponds to the latency I/II. Some lytic infection genes are expressed in EBVaGCs tissues. BARF1 and BHRF1 genes may play an important role in tumorigenesis of gastric carcinoma. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
引用
收藏
页码:629 / 633
页数:5
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