Liver-specific MicroRNA miR-122 enhances the replication of hepatitis C virus in nonhepatic cells

被引：175

作者：

Chang, Jinhong ^{[1
]}

Cruo, Ju-Tao ^{[1
]}

Jiang, Dong ^{[1
]}

Guo, Haitao ^{[1
]}

Taylor, John M. ^{[3
]}

Block, Timothy M. ^{[1
,2
]}

机构：

[1] Drexel Univ, Coll Med, Dept Microbiol & Immunol, Drexel Inst Biotechnol & Virol Res, Doylestown, PA 18902 USA

[2] Hepatitis B Fdn, Inst Hepatitis & Virus Res, Doylestown, PA 18902 USA

[3] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 16期

关键词：

D O I：

10.1128/JVI.02575-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The liver-specific microRNA miR-122 has been shown to be required for the replication of hepatitis C virus (HCV) in the hepatoma cell line Huh7. The aim of this study was to test if HCV replication can be modulated by exogenously expressed miR-122 in human embryonic kidney epithelial cells (HEK-293). Our results demonstrate that miR-122 enhances the colony formation efficiency of the HCV replicon and increases the steady-state level of HCV RNA in HEK-293 cells. Therefore, we conclude that although miR-122 is not absolutely required, it greatly enhances HCV replication in nonhepatic cells.

引用

页码：8215 / 8223

页数：9

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