Photonic-crystal-fiber platform for multicolor multilabel neurophotonic studies

被引：17

作者：

Doronina-Amitonova, Lyubov V. ^{[1
]}

Fedotov, Il'ya V. ^{[1
]}

Ivashkina, Olga I. ^{[2
]}

Zots, Marina A. ^{[2
]}

Fedotov, Andrei B. ^{[1
]}

Anokhin, Konstantin V. ^{[2
]}

Zheltikov, Aleksei M. ^{[1
,3
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Phys, Ctr Int Laser, Moscow 119992, Russia

[2] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow 125009, Russia

[3] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA

来源：

APPLIED PHYSICS LETTERS | 2011年 / 98卷 / 25期

基金：

俄罗斯基础研究基金会;

关键词：

biological techniques; biological tissues; bio-optics; brain; cellular biophysics; fluorescence; fluorescence spectroscopy; holey fibres; light scattering; molecular biophysics; multiplexing; neurophysiology; optical filters; photonic band gap; photonic crystals; proteins; supercontinuum generation; SUPERCONTINUUM GENERATION; FLUORESCENT PROTEIN; OPTICAL-FIBERS; TECHNOLOGY; AIR;

D O I：

10.1063/1.3595419

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

A supercontinuum source based on a highly nonlinear photonic-crystal fiber (PCF) is combined with a hollow-core photonic-band-gap fiber (PBGF) spectral filter for a multiplex, high-repetition-rate fiber-based interrogation of fluorescent-protein neuron-activity reporters, and fluorophore conjugate antibody labels in the brain of transgenic mice. The hollow PBGF selects those parts of the supercontinuum output of the highly nonlinear PCF that can efficiently excite the fluorescence of the biomarkers but supports no guidance within the bands, where the supercontinuum light scattered by the brain tissues could hinder the detection of the fluorescence response of the biomarkers, thus allowing a high-contrast detection of well-resolved responses from each type of biomarkers. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3595419]

引用

页数：3

共 25 条

[1] An optical neural interface:: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology [J].

Aravanis, Alexander M. ;

Wang, Li-Ping ;

Zhang, Feng ;

Meltzer, Leslie A. ;

Mogri, Murtaza Z. ;

Schneider, M. Bret ;

Deisseroth, Karl .

JOURNAL OF NEURAL ENGINEERING, 2007, 4 (03) :S143-S156

[2] Millisecond-timescale, genetically targeted optical control of neural activity [J].

Boyden, ES ;

Zhang, F ;

Bamberg, E ;

Nagel, G ;

Deisseroth, K .

NATURE NEUROSCIENCE, 2005, 8 (09) :1263-1268

[3] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[4] Single-mode photonic band gap guidance of light in air [J].

Cregan, RF ;

Mangan, BJ ;

Knight, JC ;

Birks, TA ;

Russell, PS ;

Roberts, PJ ;

Allan, DC .

SCIENCE, 1999, 285 (5433) :1537-1539

[5] Tailoring the soliton output of a photonic crystal fiber for enhanced two-photon excited luminescence response from fluorescent protein biomarkers and neuron activity reporters [J].

Doronina, Lyubov V. ;

Fedotov, Il'ya V. ;

Voronin, Aleksandr A. ;

Ivashkina, Olga I. ;

Zots, Marina A. ;

Anokhin, Konstantin V. ;

Rostova, Ekaterina ;

Fedotov, Andrei B. ;

Zheltikov, Aleksei M. .

OPTICS LETTERS, 2009, 34 (21) :3373-3375

[6] Fiber-optic probes for in vivo depth-resolved neuron-activity mapping [J].

Doronina-Amitonova, Lyubov V. ;

Fedotov, Il'ya V. ;

Ivashkina, Olga I. ;

Zots, Marina A. ;

Fedotov, Andrei B. ;

Anokhin, Konstantin V. ;

Zheltikov, Aleksei M. .

JOURNAL OF BIOPHOTONICS, 2010, 3 (10-11) :660-669

[7] Supercontinuum generation in photonic crystal fiber [J].

Dudley, John M. ;

Genty, Goery ;

Coen, Stephane .

REVIEWS OF MODERN PHYSICS, 2006, 78 (04) :1135-1184

[8] Pathfinding of olfactory neuron axons to stereotyped glomerular targets revealed by dynamic imaging in living zebrafish embryos [J].

Dynes, JL ;

Ngai, J .

NEURON, 1998, 20 (06) :1081-1091

[9] High-speed, miniaturized fluorescence microscopy in freely moving mice [J].

Flusberg, Benjamin A. ;

Nimmerjahn, Axel ;

Cocker, Eric D. ;

Mukamel, Eran A. ;

Barretto, Robert P. J. ;

Ko, Tony H. ;

Burns, Laurie D. ;

Jung, Juergen C. ;

Schnitzer, Mark J. .

NATURE METHODS, 2008, 5 (11) :935-938

[10] Ultrafast optogenetic control [J].

Gunaydin, Lisa A. ;

Yizhar, Ofer ;

Berndt, Andre ;

Sohal, Vikaas S. ;

Deisseroth, Karl ;

Hegemann, Peter .

NATURE NEUROSCIENCE, 2010, 13 (03) :387-U27

← 1 2 3 →