Determination of a novel bitter amino acid, pulcherrimine, in the gonad of the green sea urchin Hemicentrotus pulcherrimus

被引：23

作者：

Murata, Y ^{[1
]}

Sata, NU ^{[1
]}

Yokoyama, M ^{[1
]}

Kuwahara, R ^{[1
]}

Kaneniwa, M ^{[1
]}

Oohara, I ^{[1
]}

机构：

[1] Natl Res Inst Fisheries Sci, Marine Biochem Div, Kanagawa 2368648, Japan

来源：

FISHERIES SCIENCE | 2001年 / 67卷 / 02期

关键词：

amino acid; bitterness; dimethylaminoazobenzenesulfonyl chloride; gonad; green sea urchin; Hemicentrotus pulcherrimus; ovary; testis;

D O I：

10.1046/j.1444-2906.2001.00234.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A novel bitter amino acid, pulcherrimine was analyzed by reverse-phase high-performance liquid chromatography using a linear gradient elution consisting of methanol/aqueous/acetic acid and methanol/acetic acid mixture, after being labeled with dimethylaminoazobenzenesulfonyl chloride (Dabs-Cl). Linearity of the calibration curve for pulcherrimine was high (r=0.994) in the tested range from 0 to 4 mug/mL. By this method, the pulcherrimine content in the ovary and testis of the green sea urchin Hemicentrotus pulcherrimus, collected in the sea off Iwaki in November 1998, was analyzed. The mean pulcherrimine content in the ovary was 1.37 mg/100 g. In contrast, no pulcherrimine was detected in all testes examined. Correlation between the bitterness and the pulcherrimine content was statistically significant. These results strongly support that pulcherrimine is a bitter principle in the green sea urchin ovaries.

引用

页码：341 / 345

页数：5

共 12 条

[1] SEPARATION OF AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BAYER, E ;

GROM, E ;

KALTENEGGER, B ;

UHMANN, R .

ANALYTICAL CHEMISTRY, 1976, 48 (08) :1106-1109

[2] ORTHO-PHTHALALDEHYDE - FLUOROGENIC DETECTION OF PRIMARY AMINES IN PICOMOLE RANGE - COMPARISON WITH FLUORESCAMINE AND NINHYDRIN [J].

BENSON, JR ;

HARE, PE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (02) :619-622

[3] A COMPLETE SEPARATION OF DIMETHYLAMINOAZOBENZENESULPHONYL-AMINO ACIDS - AMINO-ACID-ANALYSIS WITH LOW NANOGRAM AMOUNTS OF POLYPEPTIDE WITH DIMETHYLAMINOAZOBENZENESULPHONYL CHLORIDE [J].

CHANG, JY ;

KNECHT, R ;

BRAUN, DG .

BIOCHEMICAL JOURNAL, 1982, 203 (03) :803-806

[4] AMINO-ACID-ANALYSIS AT THE PICOMOLE LEVEL - APPLICATION TO THE C-TERMINAL SEQUENCE-ANALYSIS OF POLYPEPTIDES [J].

CHANG, JY ;

KNECHT, R ;

BRAUN, DG .

BIOCHEMICAL JOURNAL, 1981, 199 (03) :547-555

[5]

FUJI AKIRA, 1960, BULL FAC FISH HOKKAIDO UNIV, V11, P1

[6] CHROMOPHORIC LABELING OF AMINO-ACIDS WITH 4-DIMETHYLAMINOAZOBENZENE-4'-SULFONYL CHLORIDE [J].

LIN, JK ;

CHANG, JY .

ANALYTICAL CHEMISTRY, 1975, 47 (09) :1634-1638

[7] SEQUENCE DETERMINATION OF PEPTIDE BY COMBINED USE OF FLUORESCEINISOTHIOCYANATE AND PHENYLISOTHIOCYANATE [J].

MURAMOTO, K ;

KAWAUCHI, H ;

TUZIMURA, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1978, 42 (08) :1559-1563

[8] THE APPLICATION OF FLUORESCEIN ISOTHIOCYANATE AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE MICROSEQUENCING OF PROTEINS AND PEPTIDES [J].

MURAMOTO, K ;

KAMIYA, H ;

KAWAUCHI, H .

ANALYTICAL BIOCHEMISTRY, 1984, 141 (02) :446-450

[9]

Murata Y, 1998, NIPPON SUISAN GAKK, V64, P477

[10] Isolation and structure of pulcherrimine, a novel bitter-tasting amino acid, from the sea urchin (Hemicentrotus pulcherrimus) ovaries [J].

Murata, Y ;

Sata, NU .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (11) :5557-5560

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