Determination of a novel bitter amino acid, pulcherrimine, in the gonad of the green sea urchin Hemicentrotus pulcherrimus

被引:23
作者
Murata, Y [1 ]
Sata, NU [1 ]
Yokoyama, M [1 ]
Kuwahara, R [1 ]
Kaneniwa, M [1 ]
Oohara, I [1 ]
机构
[1] Natl Res Inst Fisheries Sci, Marine Biochem Div, Kanagawa 2368648, Japan
关键词
amino acid; bitterness; dimethylaminoazobenzenesulfonyl chloride; gonad; green sea urchin; Hemicentrotus pulcherrimus; ovary; testis;
D O I
10.1046/j.1444-2906.2001.00234.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A novel bitter amino acid, pulcherrimine was analyzed by reverse-phase high-performance liquid chromatography using a linear gradient elution consisting of methanol/aqueous/acetic acid and methanol/acetic acid mixture, after being labeled with dimethylaminoazobenzenesulfonyl chloride (Dabs-Cl). Linearity of the calibration curve for pulcherrimine was high (r=0.994) in the tested range from 0 to 4 mug/mL. By this method, the pulcherrimine content in the ovary and testis of the green sea urchin Hemicentrotus pulcherrimus, collected in the sea off Iwaki in November 1998, was analyzed. The mean pulcherrimine content in the ovary was 1.37 mg/100 g. In contrast, no pulcherrimine was detected in all testes examined. Correlation between the bitterness and the pulcherrimine content was statistically significant. These results strongly support that pulcherrimine is a bitter principle in the green sea urchin ovaries.
引用
收藏
页码:341 / 345
页数:5
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