Effects of ropivacaine, bupivacaine and sufentanil in colon and pancreatic cancer cells in vitro

被引：42
作者：
Bundscherer, A. [1 ]
Malsy, M. [1 ]
Gebhardt, K. [1 ]
Metterlein, T. [1 ]
Plank, C. [1 ]
Wiese, C. H. [1 ]
Gruber, M. [1 ]
Graf, B. M. [1 ]
机构：
[1] Univ Regensburg, Dept Anesthesiol, D-93053 Regensburg, Germany
来源：
PHARMACOLOGICAL RESEARCH | 2015年 / 95-96卷
关键词：
Colon cancer; Pancreatic cancer; Ropivacaine; Bupivacaine; Sufentanil; Cell proliferation; Apoptosis; Cell cycle; LOCAL-ANESTHETICS; NEURAXIAL ANESTHESIA; NEONATAL NAV1.5; APOPTOSIS; SURGERY; ANALGESIA; RECURRENCE; EXPRESSION; PHARMACOKINETICS; PROLIFERATION;
D O I：
10.1016/j.phrs.2015.03.017
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The perioperative period is supposed to be a vulnerable period for cancer progression. Results of clinical studies indicate that the use of regional anesthesia can influence and improve oncological outcome of cancer patients. Uncontrolled cell proliferation and resistance to apoptotic cell death are important characteristics of solid tumors. The aim of this study was to investigate the effects of the clinically used local anesthetics ropivacaine or bupivacaine and the opioid analgesic sufentanil on cell proliferation, cell cycle distribution and apoptosis of colon (HT 29 and SW 480) and pancreatic (PaTu 8988t and PANC 1) cancer cell lines in vitro. Cell proliferation was measured by Cell Proliferation ELISA BrdU Assay. Apoptosis was analyzed by annexin V staining and cell cycle distribution was detected by flow cytometry. Ropivacaine, bupivacaine and sufentanil did not change apoptosis rate and cell cycle distribution in clinically concentration. Only high concentrations of ropivacaine or bupivacaine revealed antiproliferative potency. Protective effects of epidural anesthesia observed in clinical studies seem not to be based on direct effects of these drugs on cancer cells. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：126 / 131
页数：6
相关论文
共 37 条
  • [21] Ectopic expression of clusterin/apolipoprotein J or Bcl-2 decreases the sensitivity of HaCaT cells to toxic effects of ropivacaine
    Kontargiris, E
    Kolettas, E
    Vadalouca, A
    Trougakos, IP
    Gonos, ES
    Kalfakakou, V
    [J]. CELL RESEARCH, 2004, 14 (05) : 415 - 422
  • [22] The Relationship Between Neuraxial Anesthesia and Advanced Ovarian Cancer-Related Outcomes in the Chilean Population
    Lacassie, Hector J.
    Cartagena, Jaime
    Branes, Jorge
    Assel, Melissa
    Echevarria, Ghislaine C.
    [J]. ANESTHESIA AND ANALGESIA, 2013, 117 (03) : 653 - 660
  • [23] Only tetracaine and not other local anaesthetics induce apoptosis in rat cortical astrocytes
    Lee, W. Y.
    Park, C. J.
    Shin, T. J.
    Yum, K. W.
    Yoon, T. G.
    Seo, K. S.
    Kim, H. J.
    [J]. BRITISH JOURNAL OF ANAESTHESIA, 2009, 103 (05) : 719 - 725
  • [24] Antiproliferative Effects of Local Anesthetics on Mesenchymal Stem Cells Potential Implications for Tumor Spreading and Wound Healing
    Lucchinetti, Eliana
    Awad, Ahmed E.
    Rahman, Mamoona
    Feng, Jianhua
    Lou, Phing-How
    Zhang, Liyan
    Ionescu, Lavinia
    Lemieux, Helene
    Thebaud, Bernard
    Zaugg, Michael
    [J]. ANESTHESIOLOGY, 2012, 116 (04) : 841 - 856
  • [25] Paravertebral block with ropivacaine 0.5% versus systemic analgesia for pain relief after thoracotomy
    Marret, E
    Bazelly, B
    Taylor, G
    Lembert, N
    Deleuze, A
    Mazoit, JX
    Bonnet, FJ
    [J]. ANNALS OF THORACIC SURGERY, 2005, 79 (06) : 2109 - 2114
  • [26] Martinsson T, 1999, J PHARMACOL EXP THER, V288, P660
  • [27] Molecular pharmacology of voltage-gated sodium channel expression in metastatic disease: Clinical potential of neonatal Nav1.5 in breast cancer
    Onkal, Rustem
    Djamgoz, Mustafa B. A.
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2009, 625 (1-3) : 206 - 219
  • [28] Bupivacaine induces apoptosis via ROS in the Schwann cell line
    Park, CJ
    Park, SA
    Yoon, TG
    Lee, SJ
    Yum, KW
    Kim, HJ
    [J]. JOURNAL OF DENTAL RESEARCH, 2005, 84 (09) : 852 - 857
  • [29] Cytotoxicity of Local Anesthetics in Human Neuronal Cells
    Perez-Castro, Rosalia
    Patel, Sohin
    Garavito-Aguilar, Zayra V.
    Rosenberg, Andrew
    Recio-Pinto, Esperanza
    Zhang, Jin
    Blanck, Thomas J. J.
    Xu, Fang
    [J]. ANESTHESIA AND ANALGESIA, 2009, 108 (03) : 997 - 1007
  • [30] SHARROCK NE, 1994, ANESTH ANALG, V79, P307
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