A new headspace solid-phase microextraction coupled with gas chromatography-tandem mass spectrometry method for the simultaneous quantification of 21 microbial volatile organic compounds in urine and blood

被引:14
作者
Tabbal, Sarah [1 ,3 ]
El Aroussi, Badr [1 ,3 ]
Bouchard, Michele [1 ,3 ]
Marchand, Genevieve [2 ]
Haddad, Sami [1 ,3 ]
机构
[1] Univ Montreal, Dept Environm & Occupat Hlth DSEST, POB 6128 Succ Ctr Ville, Montreal, PQ H3C 3J7, Canada
[2] Inst Rech Robert Sauve Sante & Securite Travail I, Montreal, PQ, Canada
[3] Ctr Rech Sante Publ CReSP, Montreal, PQ, Canada
关键词
Microbial volatile organic compounds (mVOCs); Urine; Blood; HS-SPME-GC-MS/MS; Optimization; Validation; QUANTITATIVE-ANALYSIS; COMPOUNDS MVOCS; MATRIX; BIOMARKERS; CHILDREN; PROTOCOL; GROWTH; MAIZE; MS;
D O I
10.1016/j.chemosphere.2022.133901
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Mold growth can cause the development of several metabolites including microbial volatile organic compounds (mVOCs). These latter may be considered as potential biomarkers of fungal presence and have been detected in human biological matrices such as urine and blood. Exposure to molds and their metabolites (e.g., mVOCs, mycotoxins) in occupational settings, is responsible for several health effects. Thus, this exposure cannot be neglected and must be evaluated. Herein, a method has been developed to quantify 21 mVOCs in urine and human blood by headspace solid phase micro-extraction (HS-SPME) coupled with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The parameters influencing the extraction process, such as the type of fiber, the incubation and extraction time and temperature and the desorption time, have been optimized to ensure better mVOCs extraction. The developed method showed good linearity over the concentration range of the compounds (R-2 > 0.995) for all the mVOCs in all the matrices. The low limits of detection (LOD) ranging from 0.7 to 417 ng/L in urine and from 1 to 507 ng/L in blood, make the developed methods sensitive and effective for biomonitoring of exposure at low levels. Recoveries, at low and high concentrations, were between 87% and 120% in urine and between 83% and 118% in blood. The repeatability and the intermediate precision in terms of coefficients of variation (CV%) was lower than 13% and 8.58% respectively for all compounds in all matrices. These values show satisfactory accuracy and precision of the developed method. Thus, this practical, simple, and sensitive method is well suited for the simultaneous quantification of target mVOCs.
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页数:13
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