Sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation:: A comparative study of light emission and fatty acid profiles

被引：2

作者：

Gavazza, M ^{[1
]}

Marmunti, M ^{[1
]}

Catalá, A ^{[1
]}

机构：

[1] Natl Univ La Plata, Fac Ciencias Vet, Catedra Bioquim, RA-1900 La Plata, Argentina

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2005年 / 280卷 / 1-2期

关键词：

bovine; kidney; lipid peroxidation; liver; mitochondria; rat;

D O I：

10.1007/s11010-005-8233-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Much work has been carried out on non-enzymatic-induced lipid peroxidation of mitochondria obtained from different tissues of monogastric animals, but little information is available about this process in poligastric animals. Studies were carried out to determine the sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation (ascorbate-Fe2+ dependent) by comparison of light emission and fatty acid profiles. Mitochondria from both species were susceptible to lipid peroxidation. Measurements of chemiluminescence indicate that the lipid peroxidation process was more effective in mitochondria from rat liver than in the organelle obtained from bovine, whereas changes were not observed in mitochondria from rat and bovine kidney. The fatty acid composition of total lipids isolated from liver and kidney mitochondria of both species was substantially modified when subjected to non-enzymatic lipid peroxidation with a decrease of arachidonic and docosahexaenoic acids. The polyunsaturated fatty acid (PUFA) composition was higher in mitochondria obtained from rat liver (43.11 +/- 4.16) than in bovine (15.78 +/- 0.76). As a consequence, the unsaturation index (UI), was higher in mitochondria of rat liver than in bovine. Nevertheless, the PUFA composition of kidney mitochondria from both species was similar; therefore, statistically significant differences in the UI were not observed. The results suggest that mainly the PUFAs present in hepatic and kidney mitochondria were sensitive to oxidative damage. The lipid peroxidation process was more effective in rat liver mitochondria than in bovine.

引用

页码：77 / 82

页数：6

共 25 条

[11] Rat, caprine, equine and bovine erythrocyte ghosts exposed to t-butyl hydroperoxide as a model to study lipid peroxidation using a chemiluminescence assay [J].

Iglesias, BF ;

Catalá, A .

RESEARCH IN VETERINARY SCIENCE, 2005, 79 (01) :19-27

[12]

LLANILLO M, 1995, EXP HEMATOL, V23, P258

[13]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[14] REDOX CYCLING OF IRON AND LIPID-PEROXIDATION [J].

MINOTTI, G ;

AUST, SD .

LIPIDS, 1992, 27 (03) :219-226

[15] Different antioxidant effects of polyphenols on lipid peroxidation and hydroxyl radicals in the NADPH-, Fe-ascorbate- and Fe-microsomal systems [J].

Ozgová, D ;

Hermánek, J ;

Gut, I .

BIOCHEMICAL PHARMACOLOGY, 2003, 66 (07) :1127-1137

[16]

PALMER S, 1994, NUTRITION, V10, P433

[17] Low fatty acid unsaturation protects against lipid peroxidation in liver mitochondria from long-lived species: The pigeon and human case [J].

Pamplona, R ;

Prat, J ;

Cadenas, S ;

Rojas, C ;

PerezCampo, R ;

Torres, ML ;

Barja, G .

MECHANISMS OF AGEING AND DEVELOPMENT, 1996, 86 (01) :53-66

[18]

Pamplona R, 1998, J LIPID RES, V39, P1989

[19] Fatty acid profiles and non enzymatic lipid peroxidation of microsomes and mitochondria from bovine liver, kidney, lung and heart [J].

Rosa, D ;

Catala, A .

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 1998, 106 (01) :33-37

[20]

SCHNEIDER WC, 1950, J BIOL CHEM, V183, P123

← 1 2 3 →