Immobilization and Intracellular Delivery of an Anticancer Drug Using Mussel-Inspired Polydopamine Capsules

被引：308
作者：
Cui, Jiwei [1 ]
Yan, Yan [1 ]
Such, Georgina K. [1 ]
Liang, Kang [1 ]
Ochs, Christopher J. [1 ]
Postma, Almar [1 ,2 ]
Caruso, Frank [1 ]
机构：
[1] Univ Melbourne, Dept Chem & Biomol Engn, Parkville, Vic 3010, Australia
[2] CSIRO Mat Sci & Engn, Clayton, Vic 3168, Australia
来源：
BIOMACROMOLECULES | 2012年 / 13卷 / 08期
基金：
澳大利亚研究理事会;
关键词：
POLYMER CAPSULES; CARGO RELEASE; CANCER-CELLS; NANOCAPSULES; DOPAMINE; SURFACES; FUNCTIONALIZATION; COATINGS; FACILE;
D O I：
10.1021/bm300835r
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report a facile approach to immobilize pH-cleavable polymer drug conjugates in mussel inspired polydopamine (PDA) capsules for intracellular drug delivery. Our design takes advantage of the facile PDA coating to form capsules, the chemical reactivity of PDA films, and the acid-labile groups in polymer side chains for sustained pH-induced drug release. The anticancer drug doxorubicin (Dox) was conjugated to thiolated poly(methacrylic acid) (PMA(SH)) with a PH-cleavable hydrazone bond, and then immobilized in PDA capsules via robust thiol-catechol reactions between the polymer-drug conjugate and capsule walls. The loaded Dox showed limited release at physiological pH but significant release (over 85%) at endosomal/lysosomal pH. Cell viability assays showed that Dox-loaded PDA capsules enhanced the efficacy of eradicating HeLa cancer cells compared with free drug under the same assay conditions. The reported method provides a new platform for the application of stimuli responsive PDA capsules as drug delivery systems.
引用
收藏
页码：2225 / 2228
页数：4
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