Genotoxicity of Euphorbia hirta: An Allium cepa Assay

被引：87

作者：

Ping, Kwan Yuet ^{[1
]}

Darah, Ibrahim ^{[2
]}

Yusuf, Umi Kalsom ^{[3
]}

Yeng, Chen ^{[4
,5
]}

Sasidharan, Sreenivasan ^{[1
]}

机构：

[1] Univ Sains Malaysia, Inst Res Mol Med INFORMM, George Town 11800, Malaysia

[2] Univ Sains Malaysia, Sch Biol Sci, Minden 11800, Penang, Malaysia

[3] Univ Putra Malaysia, Fac Sci, Dept Biol, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Malaya, Fac Dent, OCRCC, Kuala Lumpur 50603, Malaysia

[5] Univ Malaya, Fac Dent, Dent Res & Training Unit, Kuala Lumpur 50603, Malaysia

来源：

MOLECULES | 2012年 / 17卷 / 07期

关键词：

genotoxicity; Allium cepa; ethylmethane sulfonate; mitotic index; chromosome aberrations; MEDICINAL-PLANTS; ABERRATIONS; MITOSIS; WATER;

D O I：

10.3390/molecules17077782

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential genotoxic effects of methanolic extracts of Euphorbia hirta which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using Allium cepa assay. The extracts of 125, 250, 500 and 1,000 mu g/mL were tested on root meristems of A. cepa. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of E. hirta extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of E. hirta exerted significant genotoxic and mitodepressive effects at 1,000 mu g/mL.

引用

页码：7782 / 7791

页数：10

共 19 条

[1] Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn [J].

Akinboro, A. ;

Bakare, A. A. .

JOURNAL OF ETHNOPHARMACOLOGY, 2007, 112 (03) :470-475

[2] Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes [J].

Carilta, R. ;

Marin-Morales, M. A. .

CHEMOSPHERE, 2008, 72 (05) :722-725

[3]

Christopher H. B., 1988, CYTOLOGIA, V54, P203

[4] THE ALLIUM TEST AS A STANDARD IN ENVIRONMENTAL MONITORING [J].

FISKESJO, G .

HEREDITAS, 1985, 102 (01) :99-112

[5] CHROMOSOME ABERRATION ASSAYS IN ALLIUM [J].

GRANT, WF .

MUTATION RESEARCH, 1982, 99 (03) :273-291

[6] EFFECT OF MORPHINE SULFATE ON MITOSIS OF ALLIUM-CEPA L ROOT TIPS [J].

KABARITY, A ;

ELBAYOUM.A ;

HABIB, AA .

BIOLOGIA PLANTARUM, 1974, 16 (04) :275-&

[7]

KLASTERSKA I, 1976, HEREDITAS, V83, P153

[8] ANALGESIC, ANTIPYRETIC AND ANTIINFLAMMATORY PROPERTIES OF EUPHORBIA-HIRTA [J].

LANHERS, MC ;

FLEURENTIN, J ;

DORFMAN, P ;

MORTIER, F ;

PELT, JM .

PLANTA MEDICA, 1991, 57 (03) :225-231

[9] Allium cepa test in environmental monitoring: A review on its application [J].

Leme, Daniela Morais ;

Marin-Morales, Maria Aparecida .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2009, 682 (01) :71-81

[10] EFFECTS OF ETHIDIUM-BROMIDE ON MITOSIS AND CHROMOSOMES - POSSIBLE MATERIAL BASIS FOR CHROMOSOME STICKINESS [J].

MCGILL, M ;

PATHAK, S ;

HSU, TC .

CHROMOSOMA, 1974, 47 (02) :157-167

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