Architectural limits on split genes

被引:134
作者
Sterner, DA
Carlo, T
Berget, SM
机构
[1] BAYLOR COLL MED, VERNA & MARRS MCLEAN DEPT BIOCHEM, HOUSTON, TX 77030 USA
[2] BAYLOR COLL MED, CELL & MOL BIOL PROGRAM, HOUSTON, TX 77030 USA
关键词
D O I
10.1073/pnas.93.26.15081
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Exon/intron architecture varies across the eukaryotic kingdom with large introns and small exons the rule in vertebrates and the opposite in lower eukaryotes. To investigate the relationship between exon and intron size in pre-mRNA processing, internally expanded exons were placed in vertebrate genes with small and large introns, Both exon and intron size influenced splicing phenotype, Intron size dictated if large exons were efficiently recognized, When introns were large, large exons were skipped; when introns were small, the same large exons were included, Thus, large exons were incompatible for splicing if and only if they were flanked by large introns. Both intron and exon size became problematic at approximate to 500 nt, although both exon and intron sequence influenced the size at which exons and introns failed to be recognized, These results indicate that present-day gene architecture reflects at least in part limitations on exon recognition, Furthermore, these results strengthen models that invoke pairing of splice sites during recognition of pre-mRNAs, and suggest that vertebrate consensus sequences support pairing across either introns or exons.
引用
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页码:15081 / 15085
页数:5
相关论文
共 18 条
[11]   Initial splice-site recognition and pairing during pre-mRNA splicing [J].
Reed, R .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1996, 6 (02) :215-220
[12]   EXON DEFINITION MAY FACILITATE SPLICE SITE SELECTION IN RNAS WITH MULTIPLE EXONS [J].
ROBBERSON, BL ;
COTE, GJ ;
BERGET, SM .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (01) :84-94
[13]  
RUBY SW, 1991, TRENDS GENET, V7, P79
[14]   INVIVO RECOGNITION OF A VERTEBRATE MINI-EXON AS AN EXON-INTRON-EXON UNIT [J].
STERNER, DA ;
BERGET, SM .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (05) :2677-2687
[15]   ALTERNATIVE SPLICING IN THE NEURAL CELL-ADHESION MOLECULE PRE-MESSENGER-RNA - REGULATION OF EXON-18 SKIPPING DEPENDS ON THE 5'-SPLICE SITE [J].
TACKE, R ;
GORIDIS, C .
GENES & DEVELOPMENT, 1991, 5 (08) :1416-1429
[16]   INTRON DEFINITION IN SPLICING OF SMALL DROSOPHILA INTRONS [J].
TALERICO, M ;
BERGET, SM .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :3434-3445
[17]   A SPLICING ENHANCER COMPLEX CONTROLS ALTERNATIVE SPLICING OF DOUBLESEX PREMESSENGER RNA [J].
TIAN, M ;
MANIATIS, T .
CELL, 1993, 74 (01) :105-114
[18]   2 DIFFERENT SEQUENCE ELEMENTS WITHIN EXON-4 ARE NECESSARY FOR CALCITONIN-SPECIFIC SPLICING OF THE HUMAN CALCITONIN CALCITONIN-GENE-RELATED PEPTIDE-I PRE-MESSENGER-RNA [J].
VANOERS, CCM ;
ADEMA, GJ ;
ZANDBERG, H ;
MOEN, TC ;
BAAS, PD .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (02) :951-960