Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters

被引:120
作者
Geng, Haifeng [1 ]
Bruhn, Jesper Bartholin [2 ]
Nielsen, Kristian F. [3 ]
Gram, Lone [2 ]
Belas, Robert [1 ]
机构
[1] Univ Maryland, Ctr Marine Biotechnol, Baltimore, MD 21202 USA
[2] Tech Univ Denmark, Danish Inst Fisheries Res, Dept Seafood Res, DK-2800 Lyngby, Denmark
[3] Tech Univ Denmark, Ctr Microbial Biotechnol, BioCentrum, DK-2800 Lyngby, Denmark
关键词
D O I
10.1128/AEM.02339-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The symbiotic association between the roseobacter Silicibacter sp. strain TM1040 and the dinoflagellate Pfiesteria piscicida involves bacterial chemotaxis to dinoflagellate-produced dimethylsulfoniopropionate (DMSP), DMSP demethylation, and ultimately a biofilm on the surface of the host. Biofilm formation is coincident with the production of an antibiotic and a yellow-brown pigment. In this report, we demonstrate that the antibiotic is a sulfur-containing compound, tropodithietic acid (TDA). Using random transposon insertion mutagenesis, 12 genes were identified as critical for TDA biosynthesis by the bacteria, and mutation in any one of these results in a loss of antibiotic activity (Tda(-)) and pigment production. Unexpectedly, six of the genes, referred to as tdaA-F, could not be found on the annotated TM1040 genome and were instead located on a previously unidentified plasmid (ca. 130 kb; pSTM3) that exhibited a low frequency of spontaneous loss. Homologs of tdaA and tdaB from Silicibacter sp. strain TM1040 were identified by mutagenesis in another TDA-producing roseobacter, Phaeobacter sp. strain 27-4, which also possesses two large plasmids (ca. 60 and ca. 70 kb, respectively), and tda genes were found by DNA-DNA hybridization in 88% of a diverse collection of nine roseobacters with known antibiotic activity. These data suggest that roseobacters may use a common pathway for TDA biosynthesis that involves plasmid-encoded proteins. Using metagenomic library databases and a bioinformatics approach, differences in the biogeographical distribution between the critical TDA synthesis genes were observed. The implications of these results to roseobacter survival and the interaction between TM1040 and its dinoflagellate host are discussed.
引用
收藏
页码:1535 / 1545
页数:11
相关论文
共 50 条
  • [1] Bacterial community associated with Pfiesteria-like dinoflagellate cultures
    Alavi, M
    Miller, T
    Erlandson, K
    Schneider, R
    Belas, R
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2001, 3 (06) : 380 - 396
  • [2] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [3] AUSUBEL FM, 2001, CURRENT PROTOCOLS MO
  • [4] Molecular genetics of the genus Paracoccus:: Metabolically versatile bacteria with bioenergetic flexibility
    Baker, SC
    Ferguson, SJ
    Ludwig, B
    Page, MD
    Richter, OMH
    van Spanning, RJM
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) : 1046 - +
  • [5] The three-dimensional structure of 4-hydroxybenzoyl-CoA thioesterase from Pseudomonas sp. strain CBS-3
    Benning, MM
    Wesenberg, G
    Liu, RQ
    Taylor, KL
    Dunaway-Mariano, D
    Holden, HM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (50) : 33572 - 33579
  • [6] Antibiotic production by a Roseobacter clade-affiliated species from the German Wadden Sea and its antagonistic effects on indigenous isolates
    Brinkhoff, T
    Bach, G
    Heidorn, T
    Liang, LF
    Schlingloff, A
    Simon, M
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (04) : 2560 - 2565
  • [7] Ecology, inhibitory activity, and morphogenesis of a marine antagonistic bacterium belonging to the Roseobacter clade
    Bruhn, JB
    Nielsen, KF
    Hjelm, M
    Hansen, M
    Bresciani, J
    Schulz, S
    Gram, L
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) : 7263 - 7270
  • [8] Production of antibacterial compounds and biofilm formation by Roseobacter species are influenced by culture conditions
    Bruhn, Jesper Bartholin
    Gram, Lone
    Belas, Robert
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (02) : 442 - 450
  • [9] Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage
    Buchan, A
    Collier, LS
    Neidle, EL
    Moran, MA
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (11) : 4662 - 4672
  • [10] Overview of the marine Roseobacter lineage
    Buchan, A
    González, JM
    Moran, MA
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) : 5665 - 5677