Nanobodies and recombinant binders in cell biology

被引：159

作者：

Helma, Jonas ^{[1
,2
]}

Cardoso, M. Cristina ^{[3
]}

Muyldermans, Serge ^{[4
]}

Leonhardt, Heinrich ^{[1
,2
]}

机构：

[1] Univ Munich, Dept Biol 2, D-82152 Planegg Martinsried, Germany

[2] Ctr Integrated Prot Sci Munich, D-82152 Planegg Martinsried, Germany

[3] Tech Univ Darmstadt, Dept Biol, D-64287 Darmstadt, Germany

[4] Vrije Univ Brussel, Cellular & Mol Immunol Lab, B-1050 Brussels, Belgium

来源：

JOURNAL OF CELL BIOLOGY | 2015年 / 209卷 / 05期

关键词：

ANKYRIN REPEAT PROTEINS; HEAVY-CHAIN ANTIBODIES; SINGLE-DOMAIN ANTIBODIES; CRYSTAL-STRUCTURE; LIVING CELLS; BINDING-PROTEINS; IN-VIVO; COMBINATORIAL LIBRARIES; CONFORMATIONAL-CHANGES; MOLECULAR RECOGNITION;

D O I：

10.1083/jcb.201409074

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.

引用

页码：633 / 644

页数：12

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