Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae

被引:41
作者
Ramya, Mohandass [1 ]
Anusha, Bhaskar [1 ]
Kalavathy, S. [2 ]
机构
[1] Bishop Heber Coll, Dept Biotechnol, Tiruchirappalli 620017, Tamil Nadu, India
[2] Bishop Heber Coll, Dept Environm Sci, Tiruchirappalli 620017, Tamil Nadu, India
关键词
Paenibacillus larvae; decolorization; degradation; Indigo carmine; isatin sulfonic acid;
D O I
10.1007/s10532-007-9134-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The potential of recently isolated bacteria Paenibacillus larvae for the effective decolorization of Indigo carmine was evaluated. The effects of operational parameters (temperature, pH, dye concentration, shaking/non shaking) were tested. Maximum extent of decolorization was observed when the medium was incorporated with 10 g/l of yeast extract and peptone. Decolorization was strongly inhibited at non-shaken conditions as well as incorporation of inorganic sources (sodium nitrite and ammonium chloride) in the medium. Maximum decolorization was observed at 30 degrees C (100%) and 40 degrees C (92%) at 8 h of incubation. The LC-MS and NMR analysis confirms the oxidation of Indigo carmine .The primary degradation products were found to be Isatin sulfonic acid and anthranilicacid.
引用
收藏
页码:283 / 291
页数:9
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