Anaesthetic effects of alfaxalone administered intraperitoneally alone or combined with dexmedetomidine and fentanyl in the rat

被引:23
作者
Arenillas, Mario [1 ]
Gomez de Segura, Ignacio A. [1 ]
机构
[1] Univ Complutense Madrid, Dept Anim Med & Surg, Madrid, Spain
关键词
alfaxalone; dexmedetomidine; fentanyl; intraperitoneal; anaesthesia; rat; MEDETOMIDINE; COMBINATION; PROPOFOL; PHARMACOKINETICS; CYCLODEXTRIN; BRADYCARDIA; REVERSAL;
D O I
10.1177/0023677218764214
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Alfaxalone is a neuroactive steroid used as a general anaesthetic in several species including dogs, cats, rabbits and ferrets. It has a wide margin of safety and a similar anaesthetic profile to propofol. To increase its aqueous solubility, a new formulation with cyclodextrins has been marketed recently. The objective of this study was to evaluate the anaesthetic effect of several doses of alfaxalone alone, considering differences between sexes, and alfaxalone combined with dexmedetomidine and fentanyl in the rat administered by the intraperitoneal route. A total of 40 Sprague Dawley rats, involved in three studies, were used. Firstly, 25, 35 and 45 mg kg(-1) of alfaxalone alone were tested. In a second study, alfaxalone (25 mg kg(-1), females; 75 mg kg(-1), males) was combined with dexmedetomidine (0.05 mg kg(-1)). Finally, alfaxalone (20 mg kg(-1), females; 60 mg kg(-1), males) was combined with dexmedetomidine (0.05 mg kg(-1)) and fentanyl (0.1 mg kg(-1)). Times of onset and duration of anaesthesia, and analgesia, deemed as losing of withdrawal pedal reflex, were recorded. Alfaxalone alone produced a 2 - to 3-fold longer time of anaesthesia in females, although surgical anaesthesia was not achieved in either sex. The addition of dexmedetomidine and fentanyl to alfaxalone produced a similar time of analgesia as well as increased time of anaesthesia in both sexes. In conclusion, alfaxalone produces light anaesthesia in rats, and males required a higher dose. The combination with other sedatives or analgesics, such as dexmedetomidine or fentanyl, allows a more prolonged anaesthesia with analgesic effects, potentially suitable for invasive procedures.
引用
收藏
页码:588 / 598
页数:11
相关论文
共 26 条
  • [1] Intraperitoneal propofol and propofol fentanyl, sufentanil and remifentanil combinations for mouse anaesthesia
    Alves, H. C.
    Valentim, A. M.
    Olsson, I. A. S.
    Antunes, L. M.
    [J]. LABORATORY ANIMALS, 2007, 41 (03) : 329 - 336
  • [2] Intraperitoneal anaesthesia with propofol, medetomidine and fentanyl in mice
    Alves, H. C.
    Valentim, A. M.
    Olsson, I. A. S.
    Antunes, L. M.
    [J]. LABORATORY ANIMALS, 2009, 43 (01) : 27 - 33
  • [3] Bol CJJG, 1999, J PHARMACOL EXP THER, V291, P153
  • [4] Alves HN, 2010, J AM ASSOC LAB ANIM, V49, P454
  • [5] PHARMACOLOGICAL PROPERTIES IN ANIMALS OF CT1341 - NEW STEROID ANAESTHETIC AGENT
    CHILD, KJ
    CURRIE, JP
    DAVIS, B
    DODDS, MG
    PEARCE, DR
    TWISSELL, DJ
    [J]. BRITISH JOURNAL OF ANAESTHESIA, 1971, 43 (01) : 2 - &
  • [6] Claassen V, 1994, Neglected factors in pharmacology and neuroscience research
  • [7] A NONSURFACTANT FORMULATION FOR ALFAXALONE BASED ON AN AMORPHOUS CYCLODEXTRIN - ACTIVITY STUDIES IN RATS AND DOGS
    ESTES, KS
    BREWSTER, ME
    WEBB, AI
    BODOR, N
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1990, 65 (1-2) : 101 - 107
  • [8] Effects of dexmedetomidine on respiratory mechanics and control of breathing in normal rats
    Fernandes, Fatima C.
    Ferreira, Halina C.
    Cagido, Viviane R.
    Carvalho, Giovanna M. C.
    Pereira, Leonel S.
    Faffe, Debora S.
    Zin, Walter A.
    Rocco, Patricia R. M.
    [J]. RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, 2006, 154 (03) : 342 - 350
  • [9] SEX DIFFERENCE IN RESPONSE TO ALPHAXALONE ANESTHESIA MAY BE ESTROGEN DEPENDENT
    FINK, G
    SARKAR, DK
    DOW, RC
    DICK, H
    BORTHWICK, N
    MALNICK, S
    TWINE, M
    [J]. NATURE, 1982, 298 (5871) : 270 - 272
  • [10] Anaesthetic effects in the ferret of alfaxalone alone and in combination with medetomidine or tramadol: a pilot study
    Giral, M.
    Garcia-Olmo, D. C.
    Gomez-Juarez, M.
    Gomez de Segura, I. A.
    [J]. LABORATORY ANIMALS, 2014, 48 (04) : 313 - 320