Carbohydrate-deficient transferrin evaluation in dry blood spots

被引：12

作者：

Bean, P ^{[1
]}

Sutphin, MS ^{[1
]}

Necessary, P ^{[1
]}

Agopian, MS ^{[1
]}

Liegmann, K ^{[1
]}

Ludvigsen, C ^{[1
]}

Peter, JB ^{[1
]}

机构：

[1] LABONE, OVERLAND PK, KS USA

来源：

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH | 1996年 / 20卷 / 01期

关键词：

carbohydrate-deficient transferrin; dry blood spot; isoelectric focusing; immunoblotting; laser densitometry;

D O I：

10.1111/j.1530-0277.1996.tb01044.x

中图分类号：

R194 [卫生标准、卫生检查、医药管理];

学科分类号：

摘要：

The aim of this study was to assess the performance of the isoelectric focusing/immunoblotting/laser densitometry (IEF/IB/LD) procedure to evaluate carbohydrate-deficient transferrin (CDT) derived from dry blood spots. Serum specimens obtained from insurance applicants were analyzed for CDT by IEF/IB/LD. Dry blood spots derived from 50 serum specimens were analyzed by IEF/IB/LD. A comparative analysis of these serum specimens and the paired dry blood spots by IEF/IB/LD shows al highly significant correlation of the CDT values (r = 0.94, p < 0.0001). Stability studies indicate that, under proper storage conditions (2-3 days at room temperature, 2 weeks at 4 degrees C, or frozen at or ba!low -20 degrees C indefinitely), dry blood spots can be used as a source of CDT for analysis by IEF/IB/LD, thus simplifying sampling, storage, and transportation of specimens to the testing site.

引用

页码：56 / 60

页数：5

共 12 条

[1]

ANTON R, 1994, CLIN CHEM, V40, P364

[2]

BEAN P, 1995, CLIN CHEM, V41, P858

[3]

BEAN P, 1994, CLIN CHEM, V40, P2078

[4] A NEW APPROACH TO QUANTITATE CARBOHYDRATE-DEFICIENT TRANSFERRIN ISOFORMS IN ALCOHOL ABUSERS - PARTIAL IRON SATURATION IN ISOELECTRIC FOCUSING/IMMUNOBLOTTING AND LASER DENSITOMETRY [J].