Ligase chain reaction testing of pooled urine specimens to diagnose Chlamydia trachomatis infection

被引:0
|
作者
Sánchez-Alemán, M
Gutiérrez, JP
Bertozzi, SM
Frontela-Noda, M
Guerrero-Lemus, V
Conde-González, CJ
机构
[1] Inst Nacl Salud Publ, Direcc Microbiol Med, Ctr Invest Enfermedades Infecciosas, Cuernavaca 62100, Morelos, Mexico
[2] Inst Nacl Salud Publ, Ctr Invest Sistemas Salud, Cuernavaca 62100, Morelos, Mexico
来源
REVISTA DE INVESTIGACION CLINICA-CLINICAL AND TRANSLATIONAL INVESTIGATION | 2005年 / 57卷 / 04期
关键词
sample pooling; C; trachomatis; LCR;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. The purpose of this study was to assess the utility and validity of pooling urine samples for molecular diagnosis of Chlamydia trachomatis infection. Material and methods. Of 1,220 urine samples collected from Mexican female and male adolescents, 305 pools were composed of fourth individual samples each, based on a calculation of optimal pool size. These were processed by ligase chain reaction (LCR) for the detection of C. trachomatis. Positive and grayzone pools were reanalyzed individually. Cost savings were calculated comparing actual costs of testing to the cost that would have been incurred testing all 1,220 samples individually. Results. Pools results were: 56 positive, 19 gray-zones and 230 negative. Following individual retesting of positive and gray-zone pools, 59 cases of C. trachomatis infection were identified (4.8% prevalence). Thus, a total of 601 LCR tests were performed, for a 50.4% savings considering only the direct cost of the test. Conclusions. Our experience shows that sample pooling is both a reliable and convenient tool for CT surveillance in our setting. It should be considered in other similar settings where limited resources constraint surveillance of STIs.
引用
收藏
页码:548 / 554
页数:7
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