Colloidal properties of biodegradable nanoparticles influence interaction with amyloid-β peptide

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the extracellular deposition of amyloid-beta peptides (A beta). During the past few years, promising approaches based on nanotechnologies have emerged to alter A beta aggregation and its related toxicity. This study aims to investigate the influence of the nanoparticle colloidal properties over the interaction with A beta peptide 1-42 (A beta(1-42)). Using capillary electrophoresis with laser-induced fluorescence detection, it was shown that biodegradable poly(ethylene glycol)-block-polylactide (PEG-b-PLA) nanoparticles were able to interact with A beta(1-42) peptide leading to its uptake in rather short time periods. In addition, we highlighted the crucial role of the nanocarrier colloidal properties on the uptake kinetics. Whereas nanoparticles stabilized by sodium cholate (lower size and higher negative surface charge) gave optimum uptake kinetics, nanoparticles stabilized with others surfactants presented lower interactions. In contrast, PEG density seemed to have no influence on the interaction when sodium cholate was used for the preparation. This study intends to give new insights into A beta(1-42) peptide interaction with nanoparticulate systems by helping to determine suitable nanoparticle characteristics regarding forthcoming therapeutic strategies against AD. (C) 2011 Elsevier B. V. All rights reserved.