Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster

被引：110

作者：

Bogwitz, MR ^{[1
]}

Chung, H ^{[1
]}

Magoc, L ^{[1
]}

Rigby, S ^{[1
]}

Wong, W ^{[1
]}

O'Keefe, M ^{[1
]}

McKenzie, JA ^{[1
]}

Batterham, P ^{[1
]}

Daborn, PJ ^{[1
]}

机构：

[1] Univ Melbourne, Dept Genet, Mol Sci & Biotechnol Inst Bio21, CESAR, Parkville, Vic 3010, Australia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 36期

关键词：

cytochrome P450; insecticide resistance; insect growth regulator; genetic mopping;

D O I：

10.1073/pnas.0503709102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lufenuron is an insect growth regulator insecticide mainly used for the control of the cat flea. To understand mechanisms of resistance to lufenuron, we have characterized lufenuron resistance in a natural population of Drosophila melanogaster. In this study we have used precise genetic mapping to identify a mechanism of lufenuron resistance: the overexpression of the cytochrome P450 gene Cyp12a4. Cyp12a4 is predicted to encode a mitochondrial cytochrome P450 enzyme. Expression of Cyp12a4 in D. melanogaster third-instar larvae was detected in the midgut and Malpighian tubules of both lufenuron-resistant and wild-type strains. The level of Cyp12a4 expression in the midgut is higher in the lufenuron-resistant strain than in wild-type strains. Driving the expression of Cyp12a4 in the midgut and Malpighian tubules by using the GAL4/UAS gene expression system results in lufenuron resistance, but it does not result in resistance to three other insecticide classes. Transgenic expression of Cyp12a4 in a ubiquitous expression pattern results in late embryonic lethality, suggesting that high-level ectopic expression of Cyp12a4 is detrimental to development.

引用

页码：12807 / 12812

页数：6

共 46 条

[1] Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism [J].