Determining Existance and Antibiotic Susceptibility Status of Listeria monocytogenes Isolated from Dairy Products, Serological and Moleculer Typing of the Isolates

被引:0
作者
Dumen, Emek [1 ]
Issa, Ghassan [1 ]
Ikiz, Serkan [2 ]
Bagcigil, Funda [2 ]
Ozgur, Yakut [2 ]
Kahraman, Tolga [1 ]
Ergin, Sevgi [3 ]
Yesil, Osman [1 ]
机构
[1] Istanbul Univ, Sch Vet Med, Dept Food Hyg & Technol, TR-34320 Istanbul, Turkey
[2] Istanbul Univ, Sch Vet Med, Dept Microbiol, TR-34320 Istanbul, Turkey
[3] Istanbul Univ, Cerrahpasa Sch Med, Dept Clin Microbiol, TR-34098 Istanbul, Turkey
关键词
Listeria monocytogenes; Milk; Dairy products; Serotyping; Lineage; GENETIC-CHARACTERIZATION; FOOD; RESISTANCE; STRAINS; MILK; WATER; HEAT;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study, 700 samples of dairy and its products were collected from Istanbul and Trakya region and the samples were analyzed for L. monocytogenes by using the reference methods. 20 samples of 700 were L. monocytogenes positive. The positive samples were confirmed by using PCR. Antibiotic susceptibility tests were applied to the positive samples by using 16 different kinds of antibiotic to expose the sensitivity profiles of the agents. Serotyping procedures were applied to the positive samples and all the agents were determined as L. monocytogenes serovar 4d. PCR confirmed samples were sequenced and specific DNA sequences of the agents were determined. Afterwards, Lineage groups of the positive samples were investigated and all the positive samples were exposed to be in Lineage I. The last step of our study was to compare our samples with the reference samples that are isolated from different origins which were belonged to same and different Lineages and to discuss all procedures that were applied in the study.
引用
收藏
页码:S111 / S119
页数:9
相关论文
共 30 条
  • [21] Occurrence, Antibiotic Resistance, and Population Diversity of Listeria monocytogenes Isolated From Fresh Aquatic Products in China
    Chen, Moutong
    Cheng, Jianheng
    Wu, Qingping
    Zhang, Jumei
    Chen, Yuetao
    Xue, Liang
    Lei, Tao
    Zeng, Haiyan
    Wu, Shi
    Ye, Qinghua
    Bai, Jianling
    Wang, Juan
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [22] Characterization of Listeria monocytogenes isolates from lactating dairy cows in a Wisconsin farm: Antibiotic resistance, mammalian cell infection, and effects on the fecal microbiota
    Chow, Justin T. H.
    Gall, Aaron R.
    Johnson, Alexander K.
    Huynh, TuAnh N.
    JOURNAL OF DAIRY SCIENCE, 2021, 104 (04) : 4561 - 4574
  • [23] Genetic variability among isolates of Listeria monocytogenes from food products, clinical samples and processing environments, estimated by RAPD typing
    Martinez, I
    Rorvik, LM
    Brox, V
    Lassen, J
    Seppola, M
    Gram, L
    Fonnesbech-Vogel, B
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 84 (03) : 285 - 297
  • [24] Antibiotic susceptibility and phylogenetic analyses for the origins and serotypes of Listeria monocytogenes strains isolated from ice cream and cream cakes
    Babacan, Orkun
    TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2020, 44 (05) : 1100 - 1109
  • [25] Serological and molecular ecology of Listeria monocytogenes isolates collected from 13 French pork meat salting-curing plants and their products
    Thevenot, D.
    Delignette-Muller, M. -L.
    Christieans, S.
    Leroy, S.
    Kodjo, A.
    Vernozy-Rozand, C.
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2006, 112 (02) : 153 - 161
  • [26] Antibiotic Resistance of Listeria monocytogenes Isolated from Meat-Processing Environments, Beef Products, and Clinical Cases in Brazil
    Camargo, Anderson Carlos
    Augusto de Castilho, Natalia Parma
    Lopes da Silva, Danilo Augusto
    Vallim, Deyse Christina
    Hofer, Ernesto
    Nero, Luis Augusto
    MICROBIAL DRUG RESISTANCE, 2015, 21 (04) : 458 - 462
  • [27] Antibiotic susceptibility of 259 Listeria monocytogenes strains isolated from food, food-processing plants and human samples in Germany
    Noll, Matthias
    Kleta, Sylvia
    Al Dahouk, Sascha
    JOURNAL OF INFECTION AND PUBLIC HEALTH, 2018, 11 (04) : 572 - 577
  • [28] Genetic similarity, antibiotic resistance and disinfectant susceptibility of Listeria monocytogenes isolated from chicken meat and chicken-meat processing environment in Mato Grosso, Brazil
    Carvalho, Fernanda T.
    Vieira, Bruno S.
    Vallim, Deyse C.
    Carvalho, Larrayane A.
    Carvalho, Ricardo C. T.
    Pereira, Rodrigo C. L.
    Figueiredo, Eduardo E. S.
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2019, 109 (77-82) : 77 - 82
  • [29] Identification of Listeria species and Multilocus Variable-Number Tandem Repeat Analysis (MLVA) Typing of Listeria innocua and Listeria monocytogenes Isolates from Cattle Farms and Beef and Beef-Based Products from Retail Outlets in Mpumalanga and North West Provinces, South Africa
    Manqele, Ayanda
    Gcebe, Nomakorinte
    Pierneef, Rian Ewald
    Moerane, Rebone
    Adesiyun, Abiodun Adewale
    PATHOGENS, 2023, 12 (01):
  • [30] Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland
    Pyz-Lukasik, Renata
    Pirog-Komorowska, Anna
    Policht, Agata
    FOODS, 2024, 13 (18)